3L0U

The crystal structure of unmodified tRNAPhe from Escherichia coli

3L0U の概要

• エントリーDOI: 10.2210/pdb3l0u/pdb
• 分子名称: Unmodified tRNAPhe, MAGNESIUM ION, POTASSIUM ION, ... (4 entities in total)
• 機能のキーワード: transfer rna, trna, transcript, unmodified, rna
• 由来する生物種: Escherichia coli K12
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 24573.25

構造登録者

Byrne, R.T.,Konevega, A.L.,Rodnina, M.V.,Antson, A.A. (登録日: 2009-12-10, 公開日: 2010-03-16, 最終更新日: 2023-11-01)

主引用文献

Byrne, R.T.,Konevega, A.L.,Rodnina, M.V.,Antson, A.A.
The crystal structure of unmodified tRNAPhe from Escherichia coli
Nucleic Acids Res., 38:4154-4162, 2010

PubMed Abstract: Post-transcriptional nucleoside modifications fine-tune the biophysical and biochemical properties of transfer RNA (tRNA) so that it is optimized for participation in cellular processes. Here we report the crystal structure of unmodified tRNA(Phe) from Escherichia coli at a resolution of 3 A. We show that in the absence of modifications the overall fold of the tRNA is essentially the same as that of mature tRNA. However, there are a number of significant structural differences, such as rearrangements in a triplet base pair and a widened angle between the acceptor and anticodon stems. Contrary to previous observations, the anticodon adopts the same conformation as seen in mature tRNA.

PubMed: 20203084
DOI: 10.1093/nar/gkq133

実験手法

X-RAY DIFFRACTION (3 Å)