3KZA

Crystal structure of Gyuba, a patched chimera of b-lactglobulin

3KZA の概要

• エントリーDOI: 10.2210/pdb3kza/pdb
• 分子名称: Beta-lactoglobulin (2 entities in total)
• 機能のキーワード: artificial protein, chimera protein, disulfide bond, milk protein, retinol-binding, secreted, transport, transport protein
• 由来する生物種: Equus caballus (equine, bovine); 詳細
• 細胞内の位置: Secreted: P02758
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 36621.88

構造登録者

Tsuge, H.,Ohtomo, H.,Utsunomiya, H.,Konuma, T.,Ikeguchi, M. (登録日: 2009-12-08, 公開日: 2010-12-22, 最終更新日: 2024-11-13)

主引用文献

Ohtomo, H.,Konuma, T.,Utsunoiya, H.,Tsuge, H.,Ikeguchi, M.
Structure and stability of Gyuba, a patched chimera of b-lactoglobulin
Protein Sci., 20:1867-1875, 2011

PubMed Abstract: β-lactoglobulin (LG) contains nine β-strands (strands A-I) and one α-helix. Strands A-H form a β-barrel. At neutral pH, equine LG (ELG) is monomeric, whereas bovine LG (BLG) is dimeric, and the I-strands of its two subunits form an intermolecular β-sheet. We previously constructed a chimeric ELG in which the sequence of the I-strand was replaced with that of BLG. This chimera did not dimerize. For this study, we constructed the new chimera we call Gyuba (which means cow and horse in Japanese). The amino acid sequence of Gyuba includes the sequences of the BLG secondary structures and those of the ELG loops. The crystal structure of Gyuba is very similar to that of BLG and indicates that Gyuba dimerizes via the intermolecular β-sheet formed by the two I-strands. Thus, the entire arrangement of the secondary structural elements is important for LG dimer formation.

PubMed: 21853497
DOI: 10.1002/pro.720

実験手法

X-RAY DIFFRACTION (2 Å)