3KZ3
A structure of a lambda repressor fragment mutant
Summary for 3KZ3
| Entry DOI | 10.2210/pdb3kz3/pdb |
| Related | 1LMB |
| Descriptor | Repressor protein CI (2 entities in total) |
| Functional Keywords | five helix bundle, dna-binding, repressor, transcription, transcription regulation |
| Biological source | Enterobacteria phage lambda (Bacteriophage lambda) |
| Total number of polymer chains | 2 |
| Total formula weight | 17642.24 |
| Authors | Gruebele, M.,Liu, F.,Gao, Y. (deposition date: 2009-12-07, release date: 2010-02-23, Last modification date: 2024-02-21) |
| Primary citation | Liu, F.,Gao, Y.G.,Gruebele, M. A survey of lambda repressor fragments from two-state to downhill folding. J.Mol.Biol., 397:789-798, 2010 Cited by PubMed Abstract: We survey the two-state to downhill folding transition by examining 20 lambda(6-85)* mutants that cover a wide range of stabilities and folding rates. We investigated four new lambda(6-85)* mutants designed to fold especially rapidly. Two were engineered using the core remodeling of Lim and Sauer, and two were engineered using Ferreiro et al.'s frustratometer. These proteins have probe-dependent melting temperatures as high as 80 degrees C and exhibit a fast molecular phase with the characteristic temperature dependence of the amplitude expected for downhill folding. The survey reveals a correlation between melting temperature and downhill folding previously observed for the beta-sheet protein WW domain. A simple model explains this correlation and predicts the melting temperature at which downhill folding becomes possible. An X-ray crystal structure with a 1.64-A resolution of a fast-folding mutant fragment shows regions of enhanced rigidity compared to the full wild-type protein. PubMed: 20138892DOI: 10.1016/j.jmb.2010.01.071 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.64 Å) |
Structure validation
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