3KZ3

A structure of a lambda repressor fragment mutant

3KZ3 の概要

• エントリーDOI: 10.2210/pdb3kz3/pdb
• 関連するPDBエントリー: 1LMB
• 分子名称: Repressor protein CI (2 entities in total)
• 機能のキーワード: five helix bundle, dna-binding, repressor, transcription, transcription regulation
• 由来する生物種: Enterobacteria phage lambda (Bacteriophage lambda)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 17642.24

構造登録者

Gruebele, M.,Liu, F.,Gao, Y. (登録日: 2009-12-07, 公開日: 2010-02-23, 最終更新日: 2024-02-21)

主引用文献

Liu, F.,Gao, Y.G.,Gruebele, M.
A survey of lambda repressor fragments from two-state to downhill folding.
J.Mol.Biol., 397:789-798, 2010

PubMed Abstract: We survey the two-state to downhill folding transition by examining 20 lambda(6-85)* mutants that cover a wide range of stabilities and folding rates. We investigated four new lambda(6-85)* mutants designed to fold especially rapidly. Two were engineered using the core remodeling of Lim and Sauer, and two were engineered using Ferreiro et al.'s frustratometer. These proteins have probe-dependent melting temperatures as high as 80 degrees C and exhibit a fast molecular phase with the characteristic temperature dependence of the amplitude expected for downhill folding. The survey reveals a correlation between melting temperature and downhill folding previously observed for the beta-sheet protein WW domain. A simple model explains this correlation and predicts the melting temperature at which downhill folding becomes possible. An X-ray crystal structure with a 1.64-A resolution of a fast-folding mutant fragment shows regions of enhanced rigidity compared to the full wild-type protein.

PubMed: 20138892
DOI: 10.1016/j.jmb.2010.01.071

実験手法

X-RAY DIFFRACTION (1.64 Å)