3KXC

Mutant transport protein

3KXC の概要

• エントリーDOI: 10.2210/pdb3kxc/pdb
• 関連するPDBエントリー: 2CFH
• 分子名称: Trafficking protein particle complex subunit 3, Trafficking protein particle complex subunit 6B, PALMITIC ACID, ... (4 entities in total)
• 機能のキーワード: heterodimer, endoplasmic reticulum, er-golgi transport, golgi apparatus, lipoprotein, palmitate, transport, transport protein
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Golgi apparatus, cis-Golgi network (By similarity): O43617 Q86SZ2
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 40146.13

構造登録者

Kummel, D.,Heinemann, U. (登録日: 2009-12-02, 公開日: 2010-04-21, 最終更新日: 2023-11-01)

主引用文献

Kummel, D.,Walter, J.,Heck, M.,Heinemann, U.,Veit, M.
Characterization of the self-palmitoylation activity of the transport protein particle component Bet3
Cell.Mol.Life Sci., 67:2653-2664, 2010

PubMed Abstract: Bet3, a transport protein particle component involved in vesicular trafficking, contains a hydrophobic tunnel occupied by a fatty acid linked to cysteine 68. We reported that Bet3 has a unique self-palmitoylating activity. Here we show that mutation of arginine 67 reduced self-palmitoylation of Bet3, but the effect was compensated by increasing the pH. Thus, arginine helps to deprotonate cysteine such that it could function as a nucleophile in the acylation reaction which is supported by the structural analysis of non-acylated Bet3. Using fluorescence spectroscopy we show that long-chain acyl-CoAs bind with micromolar affinity to Bet3, whereas shorter-chain acyl-CoAs do not interact. Mutants with a deleted acylation site or a blocked tunnel bind to Pal-CoA, only the latter with slightly reduced affinity. Bet3 contains three binding sites for Pal-CoA, but their number was reduced to two in the mutant with an obstructed tunnel, indicating that Bet3 contains binding sites on its surface.

PubMed: 20372964
DOI: 10.1007/s00018-010-0358-y

実験手法

X-RAY DIFFRACTION (2 Å)