3KTW

Crystal structure of the SRP19/S-domain SRP RNA complex of Sulfolobus solfataricus

3KTW の概要

• エントリーDOI: 10.2210/pdb3ktw/pdb
• 関連するPDBエントリー: 3KTV
• 分子名称: SRP RNA, Signal recognition particle 19 kDa protein, POTASSIUM ION, ... (4 entities in total)
• 機能のキーワード: ribonucleoprotein complex, rna-rna tertiary interactions, asymmetric loop, 7s rna, signal recognition particle, structural rna, rna-binding, rna-rna binding protein complex, rna/rna binding protein
• 由来する生物種: Sulfolobus solfataricus; 詳細
• 細胞内の位置: Cytoplasm (By similarity): Q980W2
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 88511.61

構造登録者

Wild, K.,Bange, G.,Bozkurt, G.,Sinning, I. (登録日: 2009-11-26, 公開日: 2010-02-16, 最終更新日: 2023-09-06)

主引用文献

Wild, K.,Bange, G.,Bozkurt, G.,Segnitz, B.,Hendricks, A.,Sinning, I.
Structural insights into the assembly of the human and archaeal signal recognition particles.
Acta Crystallogr.,Sect.D, 66:295-303, 2010

PubMed Abstract: The signal recognition particle (SRP) is a conserved ribonucleoprotein (RNP) complex that co-translationally targets membrane and secretory proteins to membranes. The assembly of the particle depends on the proper folding of the SRP RNA, which in mammalia and archaea involves an induced-fit mechanism within helices 6 and 8 in the S domain of SRP. The two helices are juxtaposed and clamped together upon binding of the SRP19 protein to their apices. In the current assembly paradigm, archaeal SRP19 causes the asymmetric loop of helix 8 to bulge out and expose the binding platform for the key player SRP54. Based on a heterologous archaeal SRP19-human SRP RNA structure, mammalian SRP19 was thought not to be able to induce this change, thus explaining the different requirements of SRP19 for SRP54 recruitment. In contrast, the crystal structures of a crenarchaeal and the all-human SRP19-SRP RNA binary complexes presented here show that the asymmetric loop is bulged out in both binary complexes. Differences in SRP assembly between mammalia and archaea are therefore independent of SRP19 and are based on differences in SRP RNA itself. A new SRP-assembly scheme is presented.

PubMed: 20179341
DOI: 10.1107/S0907444910000879

実験手法

X-RAY DIFFRACTION (3.2 Å)