3KQG

Trimeric Structure of Langerin

3KQG の概要

• エントリーDOI: 10.2210/pdb3kqg/pdb
• 分子名称: C-type lectin domain family 4 member K, CALCIUM ION (3 entities in total)
• 機能のキーワード: trimer, neck and crd, coiled coil, lectin, immune system
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Membrane ; Single-pass type II membrane protein : Q9UJ71
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 124033.84

構造登録者

Feinberg, H.,Powlesland, A.S.,Taylor, M.E.,Weis, W.I. (登録日: 2009-11-17, 公開日: 2010-02-23, 最終更新日: 2024-11-20)

主引用文献

Feinberg, H.,Powlesland, A.S.,Taylor, M.E.,Weis, W.I.
Trimeric structure of langerin.
J.Biol.Chem., 285:13285-13293, 2010

PubMed Abstract: Langerin, an endocytic receptor of Langerhans cells, binds pathogens such as human immunodeficiency virus by recognition of surface glycoconjugates and mediates their internalization into Birbeck granules. Langerin has an extracellular region consisting of a C-type carbohydrate-recognition domain (CRD) and a neck region that stabilizes formation of trimers. As in many other C-type lectins, oligomerization is required for high affinity binding to glycan ligands and is also likely to be important for determining specificity. To facilitate structural analysis of the human langerin trimer, a truncated form of the extracellular region, consisting of part of the neck and the CRD, has been characterized. Like the full-length protein, truncated langerin exists as a stable trimer in solution. Glycan array screening with the trimeric fragment shows that high mannose oligosaccharides are the best ligands for langerin. Structural analysis of the trimeric fragment of langerin confirms that the neck region forms a coiled-coil of alpha-helices. Multiple interactions between the neck region and the CRDs make the trimer a rigid unit with the three CRDs in fixed positions and the primary sugar-binding sites separated by a distance of 42 A. The fixed orientation of the sugar-binding sites in the trimer is likely to place constraints on the ligands that can be bound by langerin.

PubMed: 20181944
DOI: 10.1074/jbc.M109.086058

実験手法

X-RAY DIFFRACTION (2.3 Å)