3KJO

Crystal Structure of hPOT1V2-dTrUd(AGGGTTAG)

3KJO の概要

• エントリーDOI: 10.2210/pdb3kjo/pdb
• 関連するPDBエントリー: 1XJV 3KJP
• 分子名称: Protection of telomeres protein 1, DNA/RNA (5'-D(*T)-R(P*U)-D(P*AP*GP*GP*GP*TP*TP*AP*G)-3') (3 entities in total)
• 機能のキーワード: ob domain, protein-dna complex, protein-rna complex, alternative splicing, chromosomal protein, dna-binding, nucleus, polymorphism, telomere, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Nucleus : Q9NUX5
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 36841.77

構造登録者

Nandakumar, J.,Cech, T.R.,Podell, E.R. (登録日: 2009-11-03, 公開日: 2010-01-19, 最終更新日: 2023-09-06)

主引用文献

Nandakumar, J.,Podell, E.R.,Cech, T.R.
How telomeric protein POT1 avoids RNA to achieve specificity for single-stranded DNA.
Proc.Natl.Acad.Sci.USA, 107:651-656, 2010

PubMed Abstract: The POT1-TPP1 heterodimer, the major telomere-specific single-stranded DNA-binding protein in mammalian cells, protects chromosome ends and contributes to the regulation of telomerase. The recent discovery of telomeric RNA raises the question of how POT1 faithfully binds telomeric ssDNA and avoids illicit RNA binding that could result in its depletion from telomeres. Here we show through binding studies that a single deoxythymidine in a telomeric repeat dictates the DNA versus RNA discrimination by human POT1 and mouse POT1A. We solve the crystal structure of hPOT1 bound to DNA with a ribouridine in lieu of the critical deoxythymidine and show that this substitution results in burying the 2(')-hydroxyl group in a hydrophobic region (Phe62) of POT1 in addition to eliminating favorable hydrogen-bonding interactions at the POT1-nucleic acid interface. At amino acid 62, Phe discriminates against RNA binding and Tyr allows RNA binding. We further show that TPP1 greatly augments POT1's discrimination against RNA.

PubMed: 20080730
DOI: 10.1073/pnas.0911099107

実験手法

X-RAY DIFFRACTION (1.8 Å)