3KIC
Crystal structure of adeno-associated virus serotype 3B
Summary for 3KIC
| Entry DOI | 10.2210/pdb3kic/pdb |
| Related | 3KIE |
| Descriptor | Capsid protein VP1, 2'-DEOXYADENOSINE-5'-MONOPHOSPHATE (2 entities in total) |
| Functional Keywords | virus, capsid, parvovirus, icosahedral virus, beta barrel, single-stranded, dependovirus |
| Biological source | Adeno-associated virus 3B |
| Total number of polymer chains | 20 |
| Total formula weight | 1646368.82 |
| Authors | Lerch, T.F.,Xie, Q.,Chapman, M.S. (deposition date: 2009-11-01, release date: 2010-04-28, Last modification date: 2023-09-06) |
| Primary citation | Lerch, T.F.,Xie, Q.,Chapman, M.S. The structure of adeno-associated virus serotype 3B (AAV-3B): insights into receptor binding and immune evasion. Virology, 403:26-36, 2010 Cited by PubMed Abstract: Adeno-associated viruses (AAVs) are leading candidate vectors for human gene therapy. AAV serotypes have broad cellular tropism and use a variety of cellular receptors. AAV serotype 3 binds to heparan sulfate proteoglycan prior to cell entry and is serologically distinct from other serotypes. The capsid features that distinguish AAV-3B from other serotypes are poorly understood. The structure of AAV-3B has been determined to 2.6A resolution from twinned crystals of an infectious virus. The most distinctive structural features are located in regions implicated in receptor and antibody binding, providing insights into the cell entry mechanisms and antigenic nature of AAVs. We show that AAV-3B has a lower affinity for heparin than AAV-2, which can be rationalized by the distinct features of the AAV-3B capsid. The structure of AAV-3B provides an additional foundation for the future engineering of improved gene therapy vectors with modified receptor binding or antigenic characteristics. PubMed: 20444480DOI: 10.1016/j.virol.2010.03.027 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.603 Å) |
Structure validation
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