3KAP

Flavodoxin from Desulfovibrio desulfuricans ATCC 27774 (oxidized form)

3KAP の概要

• エントリーDOI: 10.2210/pdb3kap/pdb
• 関連するPDBエントリー: 3KAQ
• 分子名称: Flavodoxin, FLAVIN MONONUCLEOTIDE (3 entities in total)
• 機能のキーワード: flavodoxin, sulfate-reducing bacteria, electron transport, flavoprotein, fmn, transport
• 由来する生物種: Desulfovibrio desulfuricans subsp. desulfuricans str. ATCC 27774
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15903.57

構造登録者

Romero, A.,Caldeira, J.,LeGall, J.,Moura, I.,Moura, J.J.G.,Romao, M.J. (登録日: 2009-10-19, 公開日: 2009-11-24, 最終更新日: 2023-11-01)

主引用文献

Romero, A.,Caldeira, J.,Legall, J.,Moura, I.,Moura, J.J.,Romao, M.J.
Crystal structure of flavodoxin from Desulfovibrio desulfuricans ATCC 27774 in two oxidation states.
Eur.J.Biochem., 239:190-196, 1996

PubMed Abstract: The crystal structures of the flavodoxin from Desulfovibrio desulfuricans ATCC 27774 have been determined and refined for both oxidized and semi-reduced forms to final crystallographic R-factors of 17.9% (0.8-0.205-nm resolution) and 19.4% (0.8-0.215-nm resolution) respectively. Native flavodoxin crystals were grown from ammonium sulfate with cell constants a = b = 9.59 nm, c=3.37nm (oxidized crystals) and they belong to space group P3(2)21. Semireduced crystals showed some changes in cell dimensions: a = b = 9.51 nm, c=3.35 nm. The three-dimensional structures are similar to other known flavodoxins and deviations are found essentially in the isoalloxazine ring environment. Conformational changes are observed between both redox states and a flip of the Gly61-Met62 peptide bond occurs upon one-electron reduction of the FMN group. These changes influence the redox potential of the oxidized/semiquinone couple. Modulation of the redox potentials is known to be related to the association constant of the FMN group to the protein. The flavodoxin from D. desulfuricans now studied has a large span between E2 (oxidized --> semiquinone) and E1 (semiquinone --> hydroquinone) redox potentials, both these values being substantially more positive within known flavodoxins. A comparison of their FMN environment was made in both oxidation states in order to correlate functional and structural differences.

PubMed: 8706707
DOI: 10.1111/j.1432-1033.1996.0190u.x

実験手法

X-RAY DIFFRACTION (2.05 Å)