3K7Z

GCN4-Leucine zipper core mutant as N16A trigonal automatic solution

Replaces:  1RB1

Summary for 3K7Z

• Entry DOI: 10.2210/pdb3k7z/pdb
• Related: 1rb4 1rb5 1rb6
• Descriptor: General control protein GCN4 (2 entities in total)
• Functional Keywords: coiled coil, leucine zipper, structural genomics, tb structural genomics consortium, tbsgc, activator, amino-acid biosynthesis, dna-binding, nucleus, phosphoprotein, transcription, transcription regulation, dna binding protein
• Cellular location: Nucleus: P03069
• Total number of polymer chains: 3
• Total formula weight: 11887.92

Authors

Holton, J.,Alber, T.,TB Structural Genomics Consortium (TBSGC) (deposition date: 2009-10-13, release date: 2009-11-24, Last modification date: 2024-02-21)

Primary citation

Holton, J.,Alber, T.
Automated Protein Crystal Structure Determination Using Elves.
Proc.Natl.Acad.Sci.USA, 101:1537-, 2004

PubMed Abstract: Efficient determination of protein crystal structures requires automated x-ray data analysis. Here, we describe the expert system ELVES and its use to determine automatically the structure of a 12-kDa protein. Multiwavelength anomalous diffraction analysis of a selenomethionyl derivative was used to image the Asn-16-Ala variant of the GCN4 leucine zipper. In contrast to the parallel, dimeric coiled coil formed by the WT sequence, the mutant unexpectedly formed an antiparallel trimer. This structural switch reveals how avoidance of core cavities at a single site can select the native fold of a protein. All structure calculations, including indexing, data processing, locating heavy atoms, phasing by multiwavelength anomalous diffraction, model building, and refinement, were completed without human intervention. The results demonstrate the feasibility of automated methods for determining high-resolution, x-ray crystal structures of proteins.

PubMed: 14752198
DOI: 10.1073/PNAS.0306241101

Experimental method

X-RAY DIFFRACTION (1.9 Å)