3K54

Structures of human Bruton's tyrosine kinase in active and inactive conformations suggests a mechanism of activation for TEC family kinases.

Summary for 3K54

• Entry DOI: 10.2210/pdb3k54/pdb
• Descriptor: Tyrosine-protein kinase BTK, N-(2-CHLORO-6-METHYLPHENYL)-2-({6-[4-(2-HYDROXYETHYL)PIPERAZIN-1-YL]-2-METHYLPYRIMIDIN-4-YL}AMINO)-1,3-THIAZOLE-5-CARBOXAMIDE (3 entities in total)
• Functional Keywords: bruton's tyrosine kinase, btk, dasatinib, atp-binding, disease mutation, kinase, membrane, metal-binding, nucleotide-binding, nucleus, phosphoprotein, transferase, tyrosine-protein kinase
• Biological source: Homo sapiens (human)
• Cellular location: Cytoplasm (By similarity): Q06187
• Total number of polymer chains: 1
• Total formula weight: 33134.42

Authors

Marcotte, D.J.,Liu, Y.-T.,Arduini, R.M.,Hession, C.A.,Miatkowski, K.,Wildes, C.P.,Cullen, P.F.,Hopkins, B.T.,Mertsching, E.,Jenkins, T.J.,Romanowski, M.J.,Baker, D.P.,Silvian, L.F. (deposition date: 2009-10-06, release date: 2010-01-19, Last modification date: 2023-09-06)

Primary citation

Marcotte, D.J.,Liu, Y.T.,Arduini, R.M.,Hession, C.A.,Miatkowski, K.,Wildes, C.P.,Cullen, P.F.,Hong, V.,Hopkins, B.T.,Mertsching, E.,Jenkins, T.J.,Romanowski, M.J.,Baker, D.P.,Silvian, L.F.
Structures of human Bruton's tyrosine kinase in active and inactive conformations suggest a mechanism of activation for TEC family kinases.
Protein Sci., 19:429-439, 2010

PubMed Abstract: Bruton's tyrosine kinase (BTK), a member of the TEC family of kinases, plays a crucial role in B-cell maturation and mast cell activation. Although the structures of the unphosphorylated mouse BTK kinase domain and the unphosphorylated and phosphorylated kinase domains of human ITK are known, understanding the kinase selectivity profiles of BTK inhibitors has been hampered by the lack of availability of a high resolution, ligand-bound BTK structure. Here, we report the crystal structures of the human BTK kinase domain bound to either Dasatinib (BMS-354825) at 1.9 A resolution or to 4-amino-5-(4-phenoxyphenyl)-7H-pyrrolospyrimidin- 7-yl-cyclopentane at 1.6 A resolution. This data provides information relevant to the development of small molecule inhibitors targeting BTK and the TEC family of nonreceptor tyrosine kinases. Analysis of the structural differences between the TEC and Src families of kinases near the Trp-Glu-Ile motif in the N-terminal region of the kinase domain suggests a mechanism of regulation of the TEC family members.

PubMed: 20052711
DOI: 10.1002/pro.321

Experimental method

X-RAY DIFFRACTION (1.94 Å)