3K1L

Crystal Structure of FANCL

Summary for 3K1L

• Entry DOI: 10.2210/pdb3k1l/pdb
• Related PRD ID: PRD_900001
• Descriptor: Fancl, alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose, ZINC ION, ... (6 entities in total)
• Functional Keywords: ubc, ring, rwd, ligase
• Biological source: Drosophila melanogaster (Fruit fly)
• Total number of polymer chains: 2
• Total formula weight: 91633.88

Authors

Cole, A.R.,Walden, H. (deposition date: 2009-09-28, release date: 2010-02-16, Last modification date: 2024-03-20)

Primary citation

Cole, A.R.,Lewis, L.P.C.,Walden, H.
The structure of the catalytic subunit FANCL of the Fanconi anemia core complex
Nat.Struct.Mol.Biol., 17:294-298, 2010

PubMed Abstract: The Fanconi anemia (FA) pathway is activated in response to DNA damage, leading to monoubiquitination of the substrates FANCI and FANCD2 by the FA core complex. Here we report the crystal structure of FANCL, the catalytic subunit of the FA core complex, at 3.2 A. The structure reveals an architecture fundamentally different from previous sequence-based predictions. The molecule is composed of an N-terminal E2-like fold, which we term the ELF domain, a novel double-RWD (DRWD) domain, and a C-terminal really interesting new gene (RING) domain predicted to facilitate E2 binding. Binding assays show that the DRWD domain, but not the ELF domain, is responsible for substrate binding.

PubMed: 20154706
DOI: 10.1038/nsmb.1759

Experimental method

X-RAY DIFFRACTION (3.2 Å)