3K1L

Crystal Structure of FANCL

3K1L の概要

• エントリーDOI: 10.2210/pdb3k1l/pdb
• 関連するBIRD辞書のPRD_ID: PRD_900001
• 分子名称: Fancl, alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose, ZINC ION, ... (6 entities in total)
• 機能のキーワード: ubc, ring, rwd, ligase
• 由来する生物種: Drosophila melanogaster (Fruit fly)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 91633.88

構造登録者

Cole, A.R.,Walden, H. (登録日: 2009-09-28, 公開日: 2010-02-16, 最終更新日: 2024-03-20)

主引用文献

Cole, A.R.,Lewis, L.P.C.,Walden, H.
The structure of the catalytic subunit FANCL of the Fanconi anemia core complex
Nat.Struct.Mol.Biol., 17:294-298, 2010

PubMed Abstract: The Fanconi anemia (FA) pathway is activated in response to DNA damage, leading to monoubiquitination of the substrates FANCI and FANCD2 by the FA core complex. Here we report the crystal structure of FANCL, the catalytic subunit of the FA core complex, at 3.2 A. The structure reveals an architecture fundamentally different from previous sequence-based predictions. The molecule is composed of an N-terminal E2-like fold, which we term the ELF domain, a novel double-RWD (DRWD) domain, and a C-terminal really interesting new gene (RING) domain predicted to facilitate E2 binding. Binding assays show that the DRWD domain, but not the ELF domain, is responsible for substrate binding.

PubMed: 20154706
DOI: 10.1038/nsmb.1759

実験手法

X-RAY DIFFRACTION (3.2 Å)