3JUA

Structural basis of YAP recognition by TEAD4 in the Hippo pathway

3JUA の概要

• エントリーDOI: 10.2210/pdb3jua/pdb
• 分子名称: Transcriptional enhancer factor TEF-3, 65 kDa Yes-associated protein (3 entities in total)
• 機能のキーワード: tead, yap, hippo pathway, transcription, activator, dna-binding, nucleus, transcription regulation, phosphoprotein
• 由来する生物種: Mus musculus (mouse); 詳細
• 細胞内の位置: Nucleus: Q62296; Cytoplasm (By similarity): P46938
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 120893.24

構造登録者

Chen, L.,Song, H. (登録日: 2009-09-15, 公開日: 2010-02-23, 最終更新日: 2024-03-20)

主引用文献

Chen, L.,Chan, S.W.,Zhang, X.,Walsh, M.,Lim, C.J.,Hong, W.,Song, H.
Structural basis of YAP recognition by TEAD4 in the hippo pathway.
Genes Dev., 24:290-300, 2010

PubMed Abstract: The Hippo signaling pathway controls cell growth, proliferation, and apoptosis by regulating the expression of target genes that execute these processes. Acting downstream from this pathway is the YAP transcriptional coactivator, whose biological function is mediated by the conserved TEAD family transcription factors. The interaction of YAP with TEADs is critical to regulate Hippo pathway-responsive genes. Here, we describe the crystal structure of the YAP-interacting C-terminal domain of TEAD4 in complex with the TEAD-interacting N-terminal domain of YAP. The structure reveals that the N-terminal region of YAP is folded into two short helices with an extended loop containing the PXXPhiP motif in between, while the C-terminal domain of TEAD4 has an immunoglobulin-like fold. YAP interacts with TEAD4 mainly through the two short helices. Point mutations of TEAD4 indicate that the residues important for YAP interaction are required for its transforming activity. Mutagenesis reveals that the PXXPhiP motif of YAP, although making few contacts with TEAD4, is important for TEAD4 interaction as well as for the transforming activity.

PubMed: 20123908
DOI: 10.1101/gad.1865310

実験手法

X-RAY DIFFRACTION (3 Å)