3JCS

2.8 Angstrom cryo-EM structure of the large ribosomal subunit from the eukaryotic parasite Leishmania

これはPDB形式変換不可エントリーです。

3JCS の概要

• エントリーDOI: 10.2210/pdb3jcs/pdb
• EMDBエントリー: 6583
• 分子名称: 26S alpha ribosomal RNA, ribosomal protein L3, ribosomal protein L4, ... (50 entities in total)
• 機能のキーワード: leishmania donovani, ribosome, the large ribosomal subunit, eukaryotic parasite
• 由来する生物種: Leishmania donovani; 詳細
• タンパク質・核酸の鎖数: 48
• 化学式量合計: 2170515.34

構造登録者

Shalev-Benami, M.,Zhang, Y.,Matzov, D.,Halfon, Y.,Zackay, A.,Rozenberg, H.,Zimmerman, E.,Bashan, A.,Jaffe, C.L.,Yonath, A.,Skiniotis, G. (登録日: 2016-01-21, 公開日: 2016-07-20, 最終更新日: 2025-06-11)

主引用文献

Shalev-Benami, M.,Zhang, Y.,Matzov, D.,Halfon, Y.,Zackay, A.,Rozenberg, H.,Zimmerman, E.,Bashan, A.,Jaffe, C.L.,Yonath, A.,Skiniotis, G.
2.8- angstrom Cryo-EM Structure of the Large Ribosomal Subunit from the Eukaryotic Parasite Leishmania.
Cell Rep, 16:288-294, 2016

PubMed Abstract: Leishmania is a single-cell eukaryotic parasite of the Trypanosomatidae family, whose members cause an array of tropical diseases. The often fatal outcome of infections, lack of effective vaccines, limited selection of therapeutic drugs, and emerging resistant strains, underline the need to develop strategies to combat these pathogens. The Trypanosomatid ribosome has recently been highlighted as a promising therapeutic target due to structural features that are distinct from other eukaryotes. Here, we present the 2.8-Å resolution structure of the Leishmania donovani large ribosomal subunit (LSU) derived from a cryo-EM map, further enabling the structural observation of eukaryotic rRNA modifications that play a significant role in ribosome assembly and function. The structure illustrates the unique fragmented nature of leishmanial LSU rRNA and highlights the irregular distribution of rRNA modifications in Leishmania, a characteristic with implications for anti-parasitic drug development.

PubMed: 27373148
DOI: 10.1016/j.celrep.2016.06.014

実験手法

ELECTRON MICROSCOPY (2.8 Å)