3JCI

2.9 Angstrom Resolution Cryo-EM 3-D Reconstruction of Close-packed PCV2 Virus-like Particles

3JCI の概要

• エントリーDOI: 10.2210/pdb3jci/pdb
• EMDBエントリー: 6555
• 分子名称: Capsid protein (1 entity in total)
• 機能のキーワード: de novo initial model, consensus criterion, gold-standard fsc, true fsc, cross-validation, virus like particle
• 由来する生物種: Porcine circovirus-2 (PCV2)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 21999.76

構造登録者

Liu, Z.,Guo, F.,Wang, F.,Li, T.C.,Jiang, W. (登録日: 2015-12-13, 公開日: 2016-02-03, 最終更新日: 2024-02-21)

主引用文献

Liu, Z.,Guo, F.,Wang, F.,Li, T.C.,Jiang, W.
2.9 angstrom Resolution Cryo-EM 3D Reconstruction of Close-Packed Virus Particles.
Structure, 24:319-328, 2016

PubMed Abstract: Single-particle cryoelectron microscopy typically discards close-packed particle images as unusable data. Here, we report an image processing strategy and case study of obtaining near-atomic resolution 3D reconstructions from close-packed particles. Multiple independent de novo initial models were constructed to determine and cross-validate the particle parameters. The particles with consistent views were further refined including not only Euler angles and center positions but also defocus, astigmatism, beam tilt, and overall and anisotropic magnification. We demonstrated this strategy with a 2.9 Å resolution reconstruction of a 1.67 MDa virus-like particle of a circovirus, PCV2, recorded on 86 photographic films. The map resolution was further validated with a phase-randomization test and local resolution assessment, and the atomic model was validated with MolProbity and EMRinger. Close-packed virus particles were thus shown not only to be useful for high-resolution 3D reconstructions but also to allow data collection at significantly improved throughput for near-atomic resolution reconstructions.

PubMed: 26777413
DOI: 10.1016/j.str.2015.12.006

実験手法

ELECTRON MICROSCOPY (2.9 Å)