3JBF

Complex of poliovirus with VHH PVSP19B

3JBF の概要

• エントリーDOI: 10.2210/pdb3jbf/pdb
• 関連するPDBエントリー: 3JBC 3JBD 3JBE 3JBG
• EMDBエントリー: 5886 5888 6433 6434 6435
• 分子名称: Capsid protein VP1, Capsid protein VP2, Capsid protein VP3, ... (6 entities in total)
• 機能のキーワード: poliovirus, nanobodies, vhh, neutralizing antibodies, virus-immune system complex, virus/immune system
• 由来する生物種: Camelus dromedarius (Arabian camel); 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 111908.05

構造登録者

Strauss, M.,Schotte, L.,Thys, B.,Filman, D.J.,Hogle, J.M. (登録日: 2015-08-26, 公開日: 2016-01-27, 最終更新日: 2022-12-21)

主引用文献

Strauss, M.,Schotte, L.,Thys, B.,Filman, D.J.,Hogle, J.M.
Five of Five VHHs Neutralizing Poliovirus Bind the Receptor-Binding Site.
J.Virol., 90:3496-3505, 2016

PubMed Abstract: Nanobodies, or VHHs, that recognize poliovirus type 1 have previously been selected and characterized as candidates for antiviral agents or reagents for standardization of vaccine quality control. In this study, we present high-resolution cryo-electron microscopy reconstructions of poliovirus with five neutralizing VHHs. All VHHs bind the capsid in the canyon at sites that extensively overlap the poliovirus receptor-binding site. In contrast, the interaction involves a unique (and surprisingly extensive) surface for each of the five VHHs. Five regions of the capsid were found to participate in binding with all five VHHs. Four of these five regions are known to alter during the expansion of the capsid associated with viral entry. Interestingly, binding of one of the VHHs, PVSS21E, resulted in significant changes of the capsid structure and thus seems to trap the virus in an early stage of expansion.

PubMed: 26764003
DOI: 10.1128/JVI.03017-15

実験手法

ELECTRON MICROSCOPY (4.6 Å)