3JB6

In situ structures of the segmented genome and RNA polymerase complex inside a dsRNA virus

3JB6 の概要

• エントリーDOI: 10.2210/pdb3jb6/pdb
• 関連するPDBエントリー: 3JB7
• EMDBエントリー: 6404 6405 6406 6407 6408 6409
• 分子名称: RNA-dependent RNA polymerase, Viral structural protein 4, VP1 CSP, ... (4 entities in total)
• 機能のキーワード: dsrna genome organization, viral polymerase, transferase-viral protein complex, transferase/viral protein
• 由来する生物種: Bombyx mori cypovirus 1 (BmCPV); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 209008.96

構造登録者

Zhang, X.,Ding, K.,Yu, X.K.,Chang, W.,Sun, J.C.,Zhou, Z.H. (登録日: 2015-08-02, 公開日: 2015-10-28, 最終更新日: 2024-05-15)

主引用文献

Zhang, X.,Ding, K.,Yu, X.,Chang, W.,Sun, J.,Hong Zhou, Z.
In situ structures of the segmented genome and RNA polymerase complex inside a dsRNA virus.
Nature, 527:531-534, 2015

PubMed Abstract: Viruses in the Reoviridae, like the triple-shelled human rotavirus and the single-shelled insect cytoplasmic polyhedrosis virus (CPV), all package a genome of segmented double-stranded RNAs (dsRNAs) inside the viral capsid and carry out endogenous messenger RNA synthesis through a transcriptional enzyme complex (TEC). By direct electron-counting cryoelectron microscopy and asymmetric reconstruction, we have determined the organization of the dsRNA genome inside quiescent CPV (q-CPV) and the in situ atomic structures of TEC within CPV in both quiescent and transcribing (t-CPV) states. We show that the ten segmented dsRNAs in CPV are organized with ten TECs in a specific, non-symmetric manner, with each dsRNA segment attached directly to a TEC. The TEC consists of two extensively interacting subunits: an RNA-dependent RNA polymerase (RdRP) and an NTPase VP4. We find that the bracelet domain of RdRP undergoes marked conformational change when q-CPV is converted to t-CPV, leading to formation of the RNA template entry channel and access to the polymerase active site. An amino-terminal helix from each of two subunits of the capsid shell protein (CSP) interacts with VP4 and RdRP. These findings establish the link between sensing of environmental cues by the external proteins and activation of endogenous RNA transcription by the TEC inside the virus.

PubMed: 26503045
DOI: 10.1038/nature15767

実験手法

ELECTRON MICROSCOPY (3.3 Å)