3JA7

Cryo-EM structure of the bacteriophage T4 portal protein assembly at near-atomic resolution

3JA7 の概要

• エントリーDOI: 10.2210/pdb3ja7/pdb
• EMDBエントリー: 6324
• 分子名称: Portal protein gp20 (1 entity in total)
• 機能のキーワード: viral protein
• 由来する生物種: Enterobacteria phage T4
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 640300.97

構造登録者

Sun, L.,Zhang, X.,Gao, S.,Rao, P.A.,Padilla-Sanchez, V.,Chen, Z.,Sun, S.,Xiang, Y.,Subramaniam, S.,Rao, V.B.,Rossmann, M.G. (登録日: 2015-04-21, 公開日: 2015-07-22, 最終更新日: 2024-02-21)

主引用文献

Sun, L.,Zhang, X.,Gao, S.,Rao, P.A.,Padilla-Sanchez, V.,Chen, Z.,Sun, S.,Xiang, Y.,Subramaniam, S.,Rao, V.B.,Rossmann, M.G.
Cryo-EM structure of the bacteriophage T4 portal protein assembly at near-atomic resolution.
Nat Commun, 6:7548-7548, 2015

PubMed Abstract: The structure and assembly of bacteriophage T4 has been extensively studied. However, the detailed structure of the portal protein remained unknown. Here we report the structure of the bacteriophage T4 portal assembly, gene product 20 (gp20), determined by cryo-electron microscopy (cryo-EM) to 3.6 Å resolution. In addition, analysis of a 10 Å resolution cryo-EM map of an empty prolate T4 head shows how the dodecameric portal assembly interacts with the capsid protein gp23 at the special pentameric vertex. The gp20 structure also verifies that the portal assembly is required for initiating head assembly, for attachment of the packaging motor, and for participation in DNA packaging. Comparison of the Myoviridae T4 portal structure with the known portal structures of φ29, SPP1 and P22, representing Podo- and Siphoviridae, shows that the portal structure probably dates back to a time when self-replicating microorganisms were being established on Earth.

PubMed: 26144253
DOI: 10.1038/ncomms8548

実験手法

ELECTRON MICROSCOPY (3.6 Å)