3JA4

RNA-dependent RNA polymerases of transcribing cypoviruses

3JA4 の概要

• エントリーDOI: 10.2210/pdb3ja4/pdb
• 関連するPDBエントリー: 3JA5
• EMDBエントリー: 6321
• 分子名称: RNA-dependent RNA polymerase (1 entity in total)
• 機能のキーワード: rna-dependent rna polymerases, transcribing cypoviruses, icosahedral virus, transferase
• 由来する生物種: Bombyx mori cypovirus 1 (BmCPV)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 138830.11

構造登録者

Liu, H.,Cheng, L. (登録日: 2015-04-20, 公開日: 2015-10-28, 最終更新日: 2024-03-20)

主引用文献

Liu, H.,Cheng, L.
Cryo-EM shows the polymerase structures and a nonspooled genome within a dsRNA virus.
Science, 349:1347-1350, 2015

PubMed Abstract: Double-stranded RNA (dsRNA) viruses possess a segmented dsRNA genome and a number of RNA-dependent RNA polymerases (RdRps) enclosed in a capsid. Until now, the precise structures of genomes and RdRps within the capsids have been unknown. Here we report the structures of RdRps and associated RNAs within nontranscribing and transcribing cypoviruses (NCPV and TCPV, respectively), using a combination of cryo-electron microscopy (cryo-EM) and a symmetry-mismatch reconstruction method. The RdRps and associated RNAs appear to exhibit a pseudo-D3 symmetric organization in both NCPV and TCPV. However, the molecular interactions between RdRps and the genomic RNA were found to differ in these states. Our work provides insight into the mechanisms of the replication and transcription in dsRNA viruses and paves a way for structural determination of lower-symmetry complexes enclosed in higher-symmetry structures.

PubMed: 26383954
DOI: 10.1126/science.aaa4938

実験手法

ELECTRON MICROSCOPY (4.8 Å)