3J9R

Atomic structures of a bactericidal contractile nanotube in its pre- and post-contraction states

これはPDB形式変換不可エントリーです。

3J9R の概要

• エントリーDOI: 10.2210/pdb3j9r/pdb
• 関連するPDBエントリー: 3J9Q
• EMDBエントリー: 6270 6271
• 分子名称: sheath (1 entity in total)
• 機能のキーワード: pyocin, bacteriocin, sheath, tube, structural protein
• 由来する生物種: Pseudomonas aeruginosa
• タンパク質・核酸の鎖数: 36
• 化学式量合計: 1484903.95

構造登録者

Ge, P.,Scholl, D.,Leiman, P.G.,Yu, X.,Miller, J.F.,Zhou, Z.H. (登録日: 2015-02-17, 公開日: 2015-04-01, 最終更新日: 2024-02-21)

主引用文献

Ge, P.,Scholl, D.,Leiman, P.G.,Yu, X.,Miller, J.F.,Zhou, Z.H.
Atomic structures of a bactericidal contractile nanotube in its pre- and postcontraction states.
Nat.Struct.Mol.Biol., 22:377-382, 2015

PubMed Abstract: R-type pyocins are representatives of contractile ejection systems, a class of biological nanomachines that includes, among others, the bacterial type VI secretion system (T6SS) and contractile bacteriophage tails. We report atomic models of the Pseudomonas aeruginosa precontraction pyocin sheath and tube, and the postcontraction sheath, obtained by cryo-EM at 3.5-Å and 3.9-Å resolutions, respectively. The central channel of the tube is negatively charged, in contrast to the neutral and positive counterparts in T6SSs and phage tails. The sheath is interwoven by long N- and C-terminal extension arms emanating from each subunit, which create an extensive two-dimensional mesh that has the same connectivity in the extended and contracted state of the sheath. We propose that the contraction process draws energy from electrostatic and shape complementarities to insert the inner tube through bacterial cell membranes to eventually kill the bacteria.

PubMed: 25822993
DOI: 10.1038/nsmb.2995

実験手法

ELECTRON MICROSCOPY (3.9 Å)