3J9B

Electron cryo-microscopy of an RNA polymerase

3J9B の概要

• エントリーDOI: 10.2210/pdb3j9b/pdb
• EMDBエントリー: 6202
• 分子名称: Polymerase, RNA-directed RNA polymerase catalytic subunit, Polymerase basic protein 2, ... (6 entities in total)
• 機能のキーワード: influenza rdrp, single particle reconstitution, replication, rna binding protein-transferase-rna complex, rna binding protein/transferase/rna
• 由来する生物種: Influenza A virus; 詳細
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 208770.36

構造登録者

Chang, S.H.,Sun, D.P.,Liang, H.H.,Wang, J.,Li, J.,Guo, L.,Wang, X.L.,Guan, C.C.,Boruah, B.M.,Yuan, L.M.,Feng, F.,Yang, M.R.,Wojdyla, J.,Wang, J.W.,Wang, M.T.,Wang, H.W.,Liu, Y.F. (登録日: 2014-12-16, 公開日: 2015-02-18, 最終更新日: 2024-03-20)

主引用文献

Chang, S.,Sun, D.,Liang, H.,Wang, J.,Li, J.,Guo, L.,Wang, X.,Guan, C.,Boruah, B.M.,Yuan, L.,Feng, F.,Yang, M.,Wang, L.,Wang, Y.,Wojdyla, J.,Li, L.,Wang, J.,Wang, M.,Cheng, G.,Wang, H.W.,Liu, Y.
Cryo-EM Structure of Influenza Virus RNA Polymerase Complex at 4.3 angstrom Resolution.
Mol.Cell, 2015

PubMed Abstract: Replication and transcription of influenza virus genome mainly depend on its RNA-dependent RNA polymerase (RdRP), composed of the PA, PB1, and PB2 subunits. Although extensively studied, the underlying mechanism of the RdRP complex is still unclear. Here we report the biochemical characterization of influenza RdRP subcomplex comprising PA, PB1, and N terminus of PB2, which exist as dimer in solution and can assemble into a tetramer state, regulated by vRNA promoter. Using single-particle cryo-electron microscopy, we have reconstructed the RdRP tetramer complex at 4.3 Å, highlighting the assembly and interfaces between monomers within the tetrameric structure. The individual RdRP subcomplex contains all the characterized motifs and appears as a cage-like structure. High-throughput mutagenesis profiling revealed that residues involved in the oligomer state formation are critical for viral life cycle. Our results lay a solid base for understanding the mechanism of replication of influenza and other negative-stranded RNA viruses.

PubMed: 25620561
DOI: 10.1016/j.molcel.2014.12.031

実験手法

ELECTRON MICROSCOPY (4.3 Å)