3J77

Structures of yeast 80S ribosome-tRNA complexes in the rotated and non-rotated conformations (Class II - rotated ribosome with 1 tRNA)

これはPDB形式変換不可エントリーです。

3J77 の概要

• エントリーDOI: 10.2210/pdb3j77/pdb
• 関連するPDBエントリー: 3J78
• EMDBエントリー: 5976 5977
• 分子名称: 60S ribosomal protein L1, 60S ribosomal protein L10, 60S ribosomal protein L11, ... (83 entities in total)
• 機能のキーワード: 80s ribosome, kozak sequence, translation, trna, hybrid-state, classical-state, ribosome
• 由来する生物種: Saccharomyces cerevisiae (yeast); 詳細
• タンパク質・核酸の鎖数: 83
• 化学式量合計: 3210326.39

構造登録者

Svidritskiy, E.,Brilot, A.F.,Koh, C.S.,Grigorieff, N.,Korostelev, A.A. (登録日: 2014-05-29, 公開日: 2014-08-06, 最終更新日: 2024-02-21)

主引用文献

Svidritskiy, E.,Brilot, A.F.,Koh, C.S.,Grigorieff, N.,Korostelev, A.A.
Structures of Yeast 80S Ribosome-tRNA Complexes in the Rotated and Nonrotated Conformations.
Structure, 22:1210-1218, 2014

PubMed Abstract: The structural understanding of eukaryotic translation lags behind that of translation on bacterial ribosomes. Here, we present two subnanometer resolution structures of S. cerevisiae 80S ribosome complexes formed with either one or two tRNAs and bound in response to an mRNA fragment containing the Kozak consensus sequence. The ribosomes adopt two globally different conformations that are related to each other by the rotation of the small subunit. Comparison with bacterial ribosome complexes reveals that the global structures and modes of intersubunit rotation of the yeast ribosome differ significantly from those in the bacterial counterpart, most notably in the regions involving the tRNA, small ribosomal subunit, and conserved helix 69 of the large ribosomal subunit. The structures provide insight into ribosome dynamics implicated in tRNA translocation and help elucidate the role of the Kozak fragment in positioning an open reading frame during translation initiation in eukaryotes.

PubMed: 25043550
DOI: 10.1016/j.str.2014.06.003

実験手法

ELECTRON MICROSCOPY (6.2 Å)