3J47

Formation of an intricate helical bundle dictates the assembly of the 26S proteasome lid

3J47 の概要

• エントリーDOI: 10.2210/pdb3j47/pdb
• 関連するPDBエントリー: 4B4T
• EMDBエントリー: 2165
• 分子名称: 26S proteasome regulatory subunit RPN11, 26S proteasome regulatory subunit RPN8, 26S proteasome regulatory subunit RPN9, ... (8 entities in total)
• 機能のキーワード: alpha helix bundle, hybrid method, flexible fitting, protein binding
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast); 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 39828.05

構造登録者

Estrin, E.,Lopez-Blanco, J.R.,Chacon, P.,Martin, A. (登録日: 2013-06-27, 公開日: 2013-08-28, 最終更新日: 2024-02-21)

主引用文献

Estrin, E.,Lopez-Blanco, J.R.,Chacon, P.,Martin, A.
Formation of an Intricate Helical Bundle Dictates the Assembly of the 26S Proteasome Lid.
Structure, 21:1624-1635, 2013

PubMed Abstract: The 26S proteasome is the major ATP-dependent protease in eukaryotes and thus involved in regulating a diverse array of vital cellular processes. Three subcomplexes form this massive degradation machine: the lid, the base, and the core. While assembly of base and core has been well-studied, the detailed molecular mechanisms involved in formation of the nine-subunit lid remain largely unknown. Here, we reveal that helices found at the C terminus of each lid subunit form a helical bundle that directs the ordered self-assembly of the lid subcomplex. Furthermore, we use an integrative modeling approach to gain critical insights into the bundle topology and provide an important structural framework for our biochemical data. We show that the helical bundle serves as a hub through which the last-added subunit Rpn12 monitors proper lid assembly before incorporation into the proteasome. Finally, we predict that the assembly of the COP9 signalosome depends on a similar helical bundle.

PubMed: 23911091
DOI: 10.1016/j.str.2013.06.023

実験手法

ELECTRON MICROSCOPY (7.4 Å)