3J40

Validated Near-Atomic Resolution Structure of Bacteriophage Epsilon15 Derived from Cryo-EM and Modeling

「3C5B」から置き換えられました

3J40 の概要

• エントリーDOI: 10.2210/pdb3j40/pdb
• EMDBエントリー: 5678
• 分子名称: gp10, gp7 (2 entities in total)
• 機能のキーワード: capsid, accessory protein, virus
• 由来する生物種: Salmonella phage epsilon15; 詳細
• タンパク質・核酸の鎖数: 14
• 化学式量合計: 343265.22

構造登録者

Baker, M.L.,Hryc, C.F.,Zhang, Q.,Wu, W.,Jakana, J.,Haase-Pettingell, C.,Afonine, P.V.,Adams, P.D.,King, J.A.,Jiang, W.,Chiu, W. (登録日: 2013-05-30, 公開日: 2013-07-10, 最終更新日: 2024-02-21)

主引用文献

Baker, M.L.,Hryc, C.F.,Zhang, Q.,Wu, W.,Jakana, J.,Haase-Pettingell, C.,Afonine, P.V.,Adams, P.D.,King, J.A.,Jiang, W.,Chiu, W.
Validated near-atomic resolution structure of bacteriophage epsilon15 derived from cryo-EM and modeling.
Proc.Natl.Acad.Sci.USA, 110:12301-12306, 2013

PubMed Abstract: High-resolution structures of viruses have made important contributions to modern structural biology. Bacteriophages, the most diverse and abundant organisms on earth, replicate and infect all bacteria and archaea, making them excellent potential alternatives to antibiotics and therapies for multidrug-resistant bacteria. Here, we improved upon our previous electron cryomicroscopy structure of Salmonella bacteriophage epsilon15, achieving a resolution sufficient to determine the tertiary structures of both gp7 and gp10 protein subunits that form the T = 7 icosahedral lattice. This study utilizes recently established best practice for near-atomic to high-resolution (3-5 Å) electron cryomicroscopy data evaluation. The resolution and reliability of the density map were cross-validated by multiple reconstructions from truly independent data sets, whereas the models of the individual protein subunits were validated adopting the best practices from X-ray crystallography. Some sidechain densities are clearly resolved and show the subunit-subunit interactions within and across the capsomeres that are required to stabilize the virus. The presence of the canonical phage and jellyroll viral protein folds, gp7 and gp10, respectively, in the same virus suggests that epsilon15 may have emerged more recently relative to other bacteriophages.

PubMed: 23840063
DOI: 10.1073/pnas.1309947110

実験手法

ELECTRON MICROSCOPY (4.5 Å)