3J3P

Conformational Shift of a Major Poliovirus Antigen Confirmed by Immuno-Cryogenic Electron Microscopy: 135S Poliovirus and C3-Fab Complex

3J3P の概要

• エントリーDOI: 10.2210/pdb3j3p/pdb
• 関連するPDBエントリー: 3J3O
• EMDBエントリー: 5291 5292 5293
• 分子名称: C3 antibody, light chain, C3 antibody, heavy chain, Protein VP1, ... (5 entities in total)
• 機能のキーワード: 135s cell-entry intermediate particle, antibody-antigen interaction, antibody-protein interaction, picornavirus, virus-antibody interaction, antibody neutralization, neutralizing antibody interaction, conformational change, virus-immune system complex, virus/immune system
• 由来する生物種: Mus musculus (mouse); 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 137670.76

構造登録者

Lin, J.,Cheng, N.,Hogle, J.M.,Steven, A.C.,Belnap, D.M. (登録日: 2013-04-10, 公開日: 2013-07-03, 最終更新日: 2024-02-21)

主引用文献

Lin, J.,Cheng, N.,Hogle, J.M.,Steven, A.C.,Belnap, D.M.
Conformational shift of a major poliovirus antigen confirmed by immuno-cryogenic electron microscopy.
J.Immunol., 191:884-891, 2013

PubMed Abstract: Small, interfacial conformational changes occur in some Ag-Ab interactions. Using cryogenic electron microscopy (cryo-EM), we have demonstrated such changes in a major antigenic site of a poliovirus capsid protein. During cell entry, native human poliovirus (160S particle) converts to a cell entry intermediate (135S particle) and later to an RNA-released (80S) particle. By mixing particles with Fabs of the neutralizing C3 mAb, we labeled the external loop connecting the B and C β-strands (BC loop) of the capsid protein VP1 (residues 95-105) in the 160S and 135S states. We then determined three-dimensional structures by cryo-EM and enhanced their interpretability by fitting high-resolution coordinates of C3 Fab and the capsid proteins into the density maps. Binding of C3 to either 160S or 135S particles caused residues of the BC loop, located on the tip of a prominent peak known as the "mesa," to move by an estimated 5 Å. C3 Abs are neutralizing and can bind bivalently. The orientation of the bound Fabs in our reconstructions suggests that C3 neutralizes poliovirus by binding two adjacent BC loops on the same mesa and inhibiting conformational changes in the viral capsid.

PubMed: 23772035
DOI: 10.4049/jimmunol.1202014

実験手法

ELECTRON MICROSCOPY (9.1 Å)