3J35

Cryo-EM reconstruction of Dengue virus at 37 C

3J35 の概要

• エントリーDOI: 10.2210/pdb3j35/pdb
• EMDBエントリー: 5587
• 分子名称: envelope protein (1 entity in total)
• 機能のキーワード: bumpy structure, conformational change, temperature dependent, virus
• 由来する生物種: Dengue virus 2
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 131370.92

構造登録者

Zhang, X.Z.,Sheng, J.,Plevka, P.,Kuhn, R.J.,Diamond, M.S.,Rossmann, M.G. (登録日: 2013-02-24, 公開日: 2013-04-10, 最終更新日: 2024-02-21)

主引用文献

Zhang, X.,Sheng, J.,Plevka, P.,Kuhn, R.J.,Diamond, M.S.,Rossmann, M.G.
Dengue structure differs at the temperatures of its human and mosquito hosts.
Proc.Natl.Acad.Sci.USA, 110:6795-6799, 2013

PubMed Abstract: We report on a conformational transition of dengue virus when changing the temperature from that present in its mosquito vectors to that of its human host. Using cryoelectron microscopy, we show that although the virus has a smooth surface, a diameter of ∼500 Å, and little exposed membrane at room temperature, the virions have a bumpy appearance with a diameter of ∼550 Å and some exposed membrane at 37 °C. The bumpy structure at 37 °C was found to be similar to the previously predicted structure of an intermediate between the smooth mature and fusogenic forms. As humans have a body temperature of 37 °C, the bumpy form of the virus would be the form present in humans. Thus, optimal dengue virus vaccines should induce antibodies that preferentially recognize epitopes exposed on the bumpy form of the virus.

PubMed: 23569243
DOI: 10.1073/pnas.1304300110

実験手法

ELECTRON MICROSCOPY (35 Å)