3IZ3

CryoEM structure of cytoplasmic polyhedrosis virus

3IZ3 の概要

• エントリーDOI: 10.2210/pdb3iz3/pdb
• EMDBエントリー: 5233
• 分子名称: Structural protein VP3, Structural protein VP1, Viral structural protein 5 (3 entities in total)
• 機能のキーワード: cryoelectron microscopy, cytoplasmic polyhedrosis virus, reovirus, transcription, icosahedral virus, virus
• 由来する生物種: Bombyx mori cypovirus 1 (BmCPV); 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 482187.46

構造登録者

Cheng, L.,Sun, J.,Zhang, K.,Mou, Z.,Huang, X.,Ji, G.,Sun, F.,Zhang, J.,Zhu, P. (登録日: 2010-09-14, 公開日: 2011-03-16, 最終更新日: 2024-02-21)

主引用文献

Cheng, L.,Sun, J.,Zhang, K.,Mou, Z.,Huang, X.,Ji, G.,Sun, F.,Zhang, J.,Zhu, P.
Atomic model of a cypovirus built from cryo-EM structure provides insight into the mechanism of mRNA capping.
Proc.Natl.Acad.Sci.USA, 108:1373-1378, 2011

PubMed Abstract: The cytoplasmic polyhedrosis virus (CPV) from the family Reoviridae belongs to a subgroup of "turreted" reoviruses, in which the mRNA capping activity occurs in a pentameric turret. We report a full atomic model of CPV built from a 3D density map obtained using cryoelectron microscopy. The image data for the 3D reconstruction were acquired exclusively from a CCD camera. Our structure shows that the enzymatic domains of the pentameric turret of CPV are topologically conserved and that there are five unique channels connecting the guanylyltransferase and methyltransferase regions. This structural organization reveals how the channels guide nascent mRNA sequentially to guanylyltransferase, 7-N-methyltransferase, and 2'-O-methyltransferase in the turret, undergoing the highly coordinated mRNA capping activity. Furthermore, by fitting the deduced amino acid sequence of the protein VP5 to 120 large protrusion proteins on the CPV capsid shell, we confirmed that this protrusion protein is encoded by CPV RNA segment 7.

PubMed: 21220303
DOI: 10.1073/pnas.1014995108

実験手法

ELECTRON MICROSCOPY (3.9 Å)