3IVF

Crystal structure of the talin head FERM domain

3IVF の概要

• エントリーDOI: 10.2210/pdb3ivf/pdb
• 分子名称: Talin-1 (2 entities in total)
• 機能のキーワード: ferm domain, cell membrane, cell projection, cytoskeleton, membrane, phosphoprotein, cell adhesion, structural protein
• 由来する生物種: Mus musculus (Mouse)
• 細胞内の位置: Cell projection, ruffle membrane ; Peripheral membrane protein ; Cytoplasmic side : P26039
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 42931.44

構造登録者

Elliott, P.R.,Goult, B.T.,Bate, N.,Grossmann, J.G.,Roberts, G.C.K.,Critchley, D.R.,Barsukov, I.L. (登録日: 2009-09-01, 公開日: 2010-08-18, 最終更新日: 2023-11-01)

主引用文献

Elliott, P.R.,Goult, B.T.,Kopp, P.M.,Bate, N.,Grossmann, J.G.,Roberts, G.C.K.,Critchley, D.R.,Barsukov, I.L.
The Structure of the talin head reveals a novel extended conformation of the FERM domain
Structure, 18:1289-1299, 2010

PubMed Abstract: FERM domains are found in a diverse superfamily of signaling and adaptor proteins at membrane interfaces. They typically consist of three separately folded domains (F1, F2, F3) in a compact cloverleaf structure. The crystal structure of the N-terminal head of the integrin-associated cytoskeletal protein talin reported here reveals a novel FERM domain with a linear domain arrangement, plus an additional domain F0 packed against F1. While F3 binds β-integrin tails, basic residues in F1 and F2 are required for membrane association and for integrin activation. We show that these same residues are also required for cell spreading and focal adhesion assembly in cells. We suggest that the extended conformation of the talin head allows simultaneous binding to integrins via F3 and to PtdIns(4,5)P2-enriched microdomains via basic residues distributed along one surface of the talin head, and that these multiple interactions are required to stabilize integrins in the activated state.

PubMed: 20947018
DOI: 10.1016/j.str.2010.07.011

実験手法

X-RAY DIFFRACTION (1.94 Å)