3IP6

Structure of Atu2422-GABA receptor in complex with proline

3IP6 の概要

• エントリーDOI: 10.2210/pdb3ip6/pdb
• 関連するPDBエントリー: 1USK 3IP5 3IP7 3IP9 3IPA 3IPC
• 分子名称: ABC transporter, substrate binding protein (Amino acid), PROLINE, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: venus flytrap domain, transport protein
• 由来する生物種: Agrobacterium tumefaciens
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 37725.26

構造登録者

Morera, S.,Planamente, S.,Vigouroux, A. (登録日: 2009-08-17, 公開日: 2010-07-14, 最終更新日: 2023-09-06)

主引用文献

Planamente, S.,Vigouroux, A.,Mondy, S.,Nicaise, M.,Faure, D.,Morera, S.
A conserved mechanism of GABA binding and antagonism is revealed by structure-function analysis of the periplasmic binding protein Atu2422 in Agrobacterium tumefaciens.
J.Biol.Chem., 285:30294-30303, 2010

PubMed Abstract: Bacterial periplasmic binding proteins (PBPs) and eukaryotic PBP-like domains (also called as Venus flytrap modules) of G-protein-coupled receptors are involved in extracellular GABA perception. We investigated the structural and functional basis of ligand specificity of the PBP Atu2422, which is implicated in virulence and transport of GABA in the plant pathogen Agrobacterium tumefaciens. Five high-resolution x-ray structures of Atu2422 liganded to GABA, Pro, Ala, and Val and of point mutant Atu2422-F77A liganded to Leu were determined. Structural analysis of the ligand-binding site revealed two essential residues, Phe(77) and Tyr(275), the implication of which in GABA signaling and virulence was confirmed using A. tumefaciens cells expressing corresponding Atu2422 mutants. Phe(77) restricts ligand specificity to α-amino acids with a short lateral chain, which act as antagonists of GABA signaling in A. tumefaciens. Tyr(275) specifically interacts with the GABA γ-amino group. Conservation of these two key residues in proteins phylogenetically related to Atu2422 brought to light a subfamily of PBPs in which all members could bind GABA and short α-amino acids. This work led to the identification of a fingerprint sequence and structural features for defining PBPs that bind GABA and its competitors and revealed their occurrence among host-interacting proteobacteria.

PubMed: 20630861
DOI: 10.1074/jbc.M110.140715

実験手法

X-RAY DIFFRACTION (1.4 Å)