3ILD

Structure of ORF157-K57A from Acidianus filamentous virus 1

3ILD の概要

• エントリーDOI: 10.2210/pdb3ild/pdb
• 関連するPDBエントリー: 3II2 3II3 3ILE
• 分子名称: Putative uncharacterized protein, MAGNESIUM ION (2 entities in total)
• 機能のキーワード: virus, archaea, nuclease, dna binding protein
• 由来する生物種: Acidianus filamentous virus 1 (AFV-1)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 18827.81

構造登録者

Goulet, A.,Lichiere, J.,Prangishvili, D.,van Tilbeurgh, H.,Cambillau, C.,Campanacci, V. (登録日: 2009-08-07, 公開日: 2010-03-23, 最終更新日: 2023-11-01)

主引用文献

Goulet, A.,Pina, M.,Redder, P.,Prangishvili, D.,Vera, L.,Lichiere, J.,Leulliot, N.,van Tilbeurgh, H.,Ortiz-Lombardia, M.,Campanacci, V.,Cambillau, C.
ORF157 from the archaeal virus Acidianus filamentous virus 1 defines a new class of nuclease
J.Virol., 84:5025-5031, 2010

PubMed Abstract: Acidianus filamentous virus 1 (AFV1) (Lipothrixviridae) is an enveloped filamentous virus that was characterized from a crenarchaeal host. It infects Acidianus species that thrive in the acidic hot springs (>85 degrees C and pH <3) of Yellowstone National Park, WY. The AFV1 20.8-kb, linear, double-stranded DNA genome encodes 40 putative open reading frames whose sequences generally show little similarity to other genes in the sequence databases. Because three-dimensional structures are more conserved than sequences and hence are more effective at revealing function, we set out to determine protein structures from putative AFV1 open reading frames (ORF). The crystal structure of ORF157 reveals an alpha+beta protein with a novel fold that remotely resembles the nucleotidyltransferase topology. In vitro, AFV1-157 displays a nuclease activity on linear double-stranded DNA. Alanine substitution mutations demonstrated that E86 is essential to catalysis. AFV1-157 represents a novel class of nuclease, but its exact role in vivo remains to be determined.

PubMed: 20200253
DOI: 10.1128/JVI.01664-09

実験手法

X-RAY DIFFRACTION (3.1 Å)