3IJE

Crystal structure of the complete integrin alhaVbeta3 ectodomain plus an Alpha/beta transmembrane fragment

3IJE の概要

• エントリーDOI: 10.2210/pdb3ije/pdb
• 分子名称: Integrin alpha-V, Integrin beta-3, alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (8 entities in total)
• 機能のキーワード: integrin structure, activation, egf domains, flim, cell signaling, cell adhesion, cleavage on pair of basic residues, disulfide bond, glycoprotein, host-virus interaction, integrin, membrane, receptor, transmembrane, disease mutation, phosphoprotein, protein binding
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 190868.92

構造登録者

Xiong, J.-P.,Mahalingham, B.,Rui, X.,Hyman, B.T.,Goodman, S.L.,Arnaout, M.A. (登録日: 2009-08-04, 公開日: 2009-09-29, 最終更新日: 2024-11-06)

主引用文献

Xiong, J.P.,Mahalingham, B.,Alonso, J.L.,Borrelli, L.A.,Rui, X.,Anand, S.,Hyman, B.T.,Rysiok, T.,Muller-Pompalla, D.,Goodman, S.L.,Arnaout, M.A.
Crystal structure of the complete integrin alphaVbeta3 ectodomain plus an alpha/beta transmembrane fragment.
J.Cell Biol., 186:589-600, 2009

PubMed Abstract: We determined the crystal structure of 1TM-alphaVbeta3, which represents the complete unconstrained ectodomain plus short C-terminal transmembrane stretches of the alphaV and beta3 subunits. 1TM-alphaVbeta3 is more compact and less active in solution when compared with DeltaTM-alphaVbeta3, which lacks the short C-terminal stretches. The structure reveals a bent conformation and defines the alpha-beta interface between IE2 (EGF-like 2) and the thigh domains. Modifying this interface by site-directed mutagenesis leads to robust integrin activation. Fluorescent lifetime imaging microscopy of inactive full-length alphaVbeta3 on live cells yields a donor-membrane acceptor distance, which is consistent with the bent conformation and does not change in the activated integrin. These data are the first direct demonstration of conformational coupling of the integrin leg and head domains, identify the IE2-thigh interface as a critical steric barrier in integrin activation, and suggest that inside-out activation in intact cells may involve conformational changes other than the postulated switch to a genu-linear state.

PubMed: 19704023
DOI: 10.1083/jcb.200905085

実験手法

X-RAY DIFFRACTION (2.9 Å)