3IIW

Crystal structure of Eed in complex with a trimethylated histone H3K27 peptide

3IIW の概要

• エントリーDOI: 10.2210/pdb3iiw/pdb
• 関連するPDBエントリー: 2QXV 3IIY 3IJ0 3IJ1 3IJC
• 分子名称: Polycomb protein EED, Histone H3 peptide (3 entities in total)
• 機能のキーワード: wd40 domain, alternative initiation, alternative splicing, chromatin regulator, nucleus, phosphoprotein, repressor, transcription, transcription regulation, wd repeat, gene regulation
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Nucleus: O75530
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 43316.38

構造登録者

Justin, N.,Sharpe, M.L.,Martin, S.,Taylor, W.R.,De Marco, V.,Gamblin, S.J. (登録日: 2009-08-03, 公開日: 2009-09-15, 最終更新日: 2024-04-03)

主引用文献

Margueron, R.,Justin, N.,Ohno, K.,Sharpe, M.L.,Son, J.,Drury, W.J.,Voigt, P.,Martin, S.R.,Taylor, W.R.,De Marco, V.,Pirrotta, V.,Reinberg, D.,Gamblin, S.J.
Role of the polycomb protein EED in the propagation of repressive histone marks.
Nature, 461:762-767, 2009

PubMed Abstract: Polycomb group proteins have an essential role in the epigenetic maintenance of repressive chromatin states. The gene-silencing activity of the Polycomb repressive complex 2 (PRC2) depends on its ability to trimethylate lysine 27 of histone H3 (H3K27) by the catalytic SET domain of the EZH2 subunit, and at least two other subunits of the complex: SUZ12 and EED. Here we show that the carboxy-terminal domain of EED specifically binds to histone tails carrying trimethyl-lysine residues associated with repressive chromatin marks, and that this leads to the allosteric activation of the methyltransferase activity of PRC2. Mutations in EED that prevent it from recognizing repressive trimethyl-lysine marks abolish the activation of PRC2 in vitro and, in Drosophila, reduce global methylation and disrupt development. These findings suggest a model for the propagation of the H3K27me3 mark that accounts for the maintenance of repressive chromatin domains and for the transmission of a histone modification from mother to daughter cells.

PubMed: 19767730
DOI: 10.1038/nature08398

実験手法

X-RAY DIFFRACTION (1.8 Å)