3IH2

TM1030 crystallized at 323K

3IH2 の概要

• エントリーDOI: 10.2210/pdb3ih2/pdb
• 関連するPDBエントリー: 3IH3 3IH4
• 分子名称: Transcriptional regulator, TetR family (2 entities in total)
• 機能のキーワード: tm1030, transcriptional regulator, temperature, structural genomics, psi-2, protein structure initiative, midwest center for structural genomics, mcsg, dna-binding, transcription, transcription regulation, transcription regulator
• 由来する生物種: Thermotoga maritima
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 24409.08

構造登録者

Koclega, K.D.,Chruszcz, M.,Bujacz, G.,Joachimiak, A.,Minor, W.,Midwest Center for Structural Genomics (MCSG) (登録日: 2009-07-29, 公開日: 2009-08-11, 最終更新日: 2024-11-20)

主引用文献

Koclega, K.D.,Chruszcz, M.,Zimmerman, M.D.,Bujacz, G.,Minor, W.
'Hot' macromolecular crystals.
Cryst.Growth Des., 10:580-586, 2010

PubMed Abstract: Transcriptional regulator protein TM1030 from the hyperthermophile Thermotoga maritima, as well as its complex with DNA, was crystallized at a wide range of temperatures. Crystallization plates were incubated at 4, 20, 37 and 50° C over 3 weeks. The best crystals of TM1030 in complex with DNA were obtained at 4, 20 and 37° C, while TM1030 alone crystallized almost equally well in all temperatures. The crystals grown at different temperatures were used for X-ray diffraction experiments and their structures were compared. Surprisingly, the models of TM1030 obtained from crystals grown at different temperatures are similar in quality. While there are some examples of structures of proteins grown at elevated temperatures in the PDB, these temperatures appear to be underrepresented. Our studies show that crystals of some proteins may be grown and are stable at broad range of temperatures. We suggest that crystallization experiments at elevated temperatures could be used as a standard part of the crystallization protocol.

PubMed: 20161694
DOI: 10.1021/cg900971h

実験手法

X-RAY DIFFRACTION (2.3 Å)