3ICQ

Karyopherin nuclear state

3ICQ の概要

• エントリーDOI: 10.2210/pdb3icq/pdb
• 関連するPDBエントリー: 3IBV
• 分子名称: Exportin-T, GTP-binding nuclear protein GSP1/CNR1, RNA (62-MER), ... (6 entities in total)
• 機能のキーワード: karyopherin, exportin, heat repeat, trna, gtpase, rna binding protein
• 由来する生物種: Schizosaccharomyces pombe (Fission yeast); 詳細
• 細胞内の位置: Nucleus: O94258 P32835
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 304349.71

構造登録者

Cook, A.G.,Fukuhara, N.,Jinek, M.,Conti, E. (登録日: 2009-07-18, 公開日: 2009-08-25, 最終更新日: 2023-11-01)

主引用文献

Cook, A.G.,Fukuhara, N.,Jinek, M.,Conti, E.
Structures of the tRNA export factor in the nuclear and cytosolic states
Nature, 461:60-65, 2009

PubMed Abstract: Transfer RNAs are among the most ubiquitous molecules in cells, central to decoding information from messenger RNAs on translating ribosomes. In eukaryotic cells, tRNAs are actively transported from their site of synthesis in the nucleus to their site of function in the cytosol. This is mediated by a dedicated nucleo-cytoplasmic transport factor of the karyopherin-beta family (Xpot, also known as Los1 in Saccharomyces cerevisiae). Here we report the 3.2 A resolution structure of Schizosaccharomyces pombe Xpot in complex with tRNA and RanGTP, and the 3.1 A structure of unbound Xpot, revealing both nuclear and cytosolic snapshots of this transport factor. Xpot undergoes a large conformational change on binding cargo, wrapping around the tRNA and, in particular, binding to the tRNA 5' and 3' ends. The binding mode explains how Xpot can recognize all mature tRNAs in the cell and yet distinguish them from those that have not been properly processed, thus coupling tRNA export to quality control.

PubMed: 19680239
DOI: 10.1038/nature08394

実験手法

X-RAY DIFFRACTION (3.2 Å)