3IBY

Structure of cytosolic domain of L. pneumophila FeoB

3IBY の概要

• エントリーDOI: 10.2210/pdb3iby/pdb
• 関連するPDBエントリー: 3I8S 3I8X 3I92
• 分子名称: Ferrous iron transport protein B (2 entities in total)
• 機能のキーワード: g protein, g domain, iron uptake, cell inner membrane, cell membrane, gtp-binding, ion transport, iron, iron transport, membrane, nucleotide-binding, transmembrane, transport, transport protein
• 由来する生物種: Legionella pneumophila
• 細胞内の位置: Cell inner membrane ; Multi-pass membrane protein : Q8GNS3
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 113614.74

構造登録者

Petermann, N.,Hansen, G.,Hilgenfeld, R. (登録日: 2009-07-17, 公開日: 2009-10-13, 最終更新日: 2024-02-21)

主引用文献

Petermann, N.,Hansen, G.,Schmidt, C.L.,Hilgenfeld, R.
Structure of the GTPase and GDI domains of FeoB, the ferrous iron transporter of Legionella pneumophila.
Febs Lett., 584:733-738, 2010

PubMed Abstract: Prokaryotic pathogens have developed specialized mechanisms for efficient uptake of ferrous iron (Fe(2+)) from the host. In Legionella pneumophila, the causative agent of Legionnaires' disease, the transmembrane GTPase FeoB plays a key role in Fe(2+) acquisition and virulence. FeoB consists of a membrane-embedded core and an N-terminal, cytosolic region (NFeoB). Here, we report the crystal structure of NFeoB from L. pneumophila, revealing a monomeric protein comprising two separate domains with GTPase and guanine-nucleotide dissociation inhibitor (GDI) functions. The GDI domain displays a novel fold, whereas the overall structure of the GTPase domain resembles that of known G domains but is in the rarely observed nucleotide-free state.

PubMed: 20036663
DOI: 10.1016/j.febslet.2009.12.045

実験手法

X-RAY DIFFRACTION (2.5 Å)