3IBP

The Crystal Structure of the Dimerization Domain of Escherichia coli Structural Maintenance of Chromosomes Protein MukB

3IBP の概要

• エントリーDOI: 10.2210/pdb3ibp/pdb
• 分子名称: Chromosome partition protein mukB, AMMONIUM ION (3 entities in total)
• 機能のキーワード: mukb, structural maintenance of chromosomes, smc, condensin, cohesin, chromosome segregation, hinge, dimerization domain, atp-binding, cell cycle, cell division, chromosome partition, dna condensation, dna-binding, nucleotide-binding
• 由来する生物種: Escherichia coli
• 細胞内の位置: Cytoplasm, nucleoid : P22523
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 34660.67

構造登録者

Li, Y.,Schoeffler, A.J.,Berger, J.M.,Oakley, M.G. (登録日: 2009-07-16, 公開日: 2010-01-26, 最終更新日: 2024-11-20)

主引用文献

Li, Y.,Schoeffler, A.J.,Berger, J.M.,Oakley, M.G.
The crystal structure of the hinge domain of the Escherichia coli structural maintenance of chromosomes protein MukB.
J.Mol.Biol., 395:11-19, 2010

PubMed Abstract: MukB, a divergent structural maintenance of chromosomes (SMC) protein, is important for chromosomal segregation and condensation in gamma-proteobacteria. MukB and canonical SMC proteins share a characteristic five-domain structure. Globular N- and C-terminal domains interact to form an ATP-binding cassette-like ATPase or "head" domain, which is connected to a smaller dimerization or "hinge" domain by a long, antiparallel coiled coil. In addition to mediating dimerization, this hinge region has been implicated in both conformational flexibility and dynamic protein-DNA interactions. We report here the first crystallographic model of the MukB hinge domain. This model also contains approximately 20% of the coiled-coil domain, including an unusual coiled-coil deviation. These results will facilitate studies to clarify the roles of both the hinge and the coiled-coil domains in MukB function.

PubMed: 19853611
DOI: 10.1016/j.jmb.2009.10.040

実験手法

X-RAY DIFFRACTION (3.099 Å)