3I71

Ethanolamine Utilization Microcompartment Shell Subunit, EutK C-terminal domain

3I71 の概要

• エントリーDOI: 10.2210/pdb3i71/pdb
• 関連するPDBエントリー: 3I6P 3I82 3I87 3I96 3IA0
• 分子名称: Ethanolamine utilization protein eutK, CITRATE ANION (3 entities in total)
• 機能のキーワード: helix-turn-helix, unknown function
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 15879.84

構造登録者

Tanaka, S.,Sawaya, M.R.,Yeates, T.O. (登録日: 2009-07-07, 公開日: 2010-01-12, 最終更新日: 2024-02-21)

主引用文献

Tanaka, S.,Sawaya, M.R.,Yeates, T.O.
Structure and Mechanisms of a Protein-Based Organelle in Escherichia coli.
Science, 327:81-84, 2010

PubMed Abstract: Many bacterial cells contain proteinaceous microcompartments that act as simple organelles by sequestering specific metabolic processes involving volatile or toxic metabolites. Here we report the three-dimensional (3D) crystal structures, with resolutions between 1.65 and 2.5 angstroms, of the four homologous proteins (EutS, EutL, EutK, and EutM) that are thought to be the major shell constituents of a functionally complex ethanolamine utilization (Eut) microcompartment. The Eut microcompartment is used to sequester the metabolism of ethanolamine in bacteria such as Escherichia coli and Salmonella enterica. The four Eut shell proteins share an overall similar 3D fold, but they have distinguishing structural features that help explain the specific roles they play in the microcompartment. For example, EutL undergoes a conformational change that is probably involved in gating molecular transport through shell protein pores, whereas structural evidence suggests that EutK might bind a nucleic acid component. Together these structures give mechanistic insight into bacterial microcompartments.

PubMed: 20044574
DOI: 10.1126/science.1179513

実験手法

X-RAY DIFFRACTION (2.1 Å)