3I6L

Newly identified epitope N1 derived from SARS-CoV N protein complexed with HLA-A*2402

3I6L の概要

• エントリーDOI: 10.2210/pdb3i6l/pdb
• 分子名称: HLA class I histocompatibility antigen, A-24 alpha chain, Beta-2-microglobulin, Nucleoprotein peptide, ... (4 entities in total)
• 機能のキーワード: hla-a*2402, sars-cov, nucleocapsid protein, disulfide bond, glycoprotein, host-virus interaction, immune response, membrane, mhc i, transmembrane, disease mutation, glycation, immunoglobulin domain, pyrrolidone carboxylic acid, secreted, golgi apparatus, phosphoprotein, ribonucleoprotein, rna-binding, viral nucleoprotein, virion, immune system
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Membrane; Single-pass type I membrane protein: P05534; Secreted: P61769; Virion: P59595
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 44521.54

構造登録者

Liu, J.W. (登録日: 2009-07-07, 公開日: 2010-07-14, 最終更新日: 2024-10-09)

主引用文献

Liu, J.,Wu, P.,Gao, F.,Qi, J.,Kawana-Tachikawa, A.,Xie, J.,Vavricka, C.J.,Iwamoto, A.,Li, T.,Gao, G.F.
Novel immunodominant peptide presentation strategy: a featured HLA-A*2402-restricted cytotoxic T-lymphocyte epitope stabilized by intrachain hydrogen bonds from severe acute respiratory syndrome coronavirus nucleocapsid protein
J.Virol., 84:11849-11857, 2010

PubMed Abstract: Antigenic peptides recognized by virus-specific cytotoxic T lymphocytes (CTLs) are presented by major histocompatibility complex (MHC; or human leukocyte antigen [HLA] in humans) molecules, and the peptide selection and presentation strategy of the host has been studied to guide our understanding of cellular immunity and vaccine development. Here, a severe acute respiratory syndrome coronavirus (SARS-CoV) nucleocapsid (N) protein-derived CTL epitope, N1 (QFKDNVILL), restricted by HLA-A*2402 was identified by a series of in vitro studies, including a computer-assisted algorithm for prediction, stabilization of the peptide by co-refolding with HLA-A*2402 heavy chain and β(2)-microglobulin (β(2)m), and T2-A24 cell binding. Consequently, the antigenicity of the peptide was confirmed by enzyme-linked immunospot (ELISPOT), proliferation assays, and HLA-peptide complex tetramer staining using peripheral blood mononuclear cells (PBMCs) from donors who had recovered from SARS donors. Furthermore, the crystal structure of HLA-A*2402 complexed with peptide N1 was determined, and the featured peptide was characterized with two unexpected intrachain hydrogen bonds which augment the central residues to bulge out of the binding groove. This may contribute to the T-cell receptor (TCR) interaction, showing a host immunodominant peptide presentation strategy. Meanwhile, a rapid and efficient strategy is presented for the determination of naturally presented CTL epitopes in the context of given HLA alleles of interest from long immunogenic overlapping peptides.

PubMed: 20844028
DOI: 10.1128/JVI.01464-10

実験手法

X-RAY DIFFRACTION (2.4 Å)