3I5X

Structure of Mss116p bound to ssRNA and AMP-PNP

3I5X の概要

• エントリーDOI: 10.2210/pdb3i5x/pdb
• 関連するPDBエントリー: 3I5Y 3I61 3I62
• 分子名称: ATP-dependent RNA helicase MSS116, 5'-R(*UP*UP*UP*UP*UP*UP*UP*UP*UP*U)-3', PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER, ... (5 entities in total)
• 機能のキーワード: protein-rna complex, rna helicase, dead-box, atp-binding, helicase, hydrolase, mitochondrion, mrna processing, mrna splicing, nucleotide-binding, rna-binding, transit peptide, translation regulation, hydrolase-rna complex, hydrolase/rna
• 由来する生物種: Saccharomyces cerevisiae (yeast); 詳細
• 細胞内の位置: Mitochondrion matrix: P15424
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 68030.92

構造登録者

Del Campo, M.,Lambowitz, A.M. (登録日: 2009-07-06, 公開日: 2009-09-08, 最終更新日: 2024-02-21)

主引用文献

Del Campo, M.,Lambowitz, A.M.
Structure of the Yeast DEAD box protein Mss116p reveals two wedges that crimp RNA
Mol.Cell, 35:598-609, 2009

PubMed Abstract: The yeast DEAD box protein Mss116p is a general RNA chaperone that functions in mitochondrial group I and II intron splicing, translational activation, and RNA end processing. Here we determined high-resolution X-ray crystal structures of Mss116p complexed with an RNA oligonucleotide and ATP analogs AMP-PNP, ADP-BeF(3)(-), or ADP-AlF(4)(-). The structures show the entire helicase core acting together with a functionally important C-terminal extension. In all structures, the helicase core is in a closed conformation with a wedge alpha helix bending RNA 3' of the central bound nucleotides, as in previous DEAD box protein structures. Notably, Mss116p's C-terminal extension also bends RNA 5' of the central nucleotides, resulting in RNA crimping. Despite reported functional differences, we observe few structural changes in ternary complexes with different ATP analogs. The structures constrain models of DEAD box protein function and reveal a strand separation mechanism in which a protein uses two wedges to act as a molecular crimper.

PubMed: 19748356
DOI: 10.1016/j.molcel.2009.07.032

実験手法

X-RAY DIFFRACTION (1.9 Å)