3I58

Crystal structure of an O-methyltransferase (NcsB1) from neocarzinostatin biosynthesis in complex with S-adenosyl-L-homocysteine (SAH) and 2-hydroxy-7-methoxy-5-methyl naphthoic acid (NA)

3I58 の概要

• エントリーDOI: 10.2210/pdb3i58/pdb
• 関連するPDBエントリー: 3I53 3I5U 3I64
• 分子名称: O-methyltransferase, S-ADENOSYL-L-HOMOCYSTEINE, 2-hydroxy-7-methoxy-5-methyl naphthoic acid, ... (5 entities in total)
• 機能のキーワード: ternary complex, rossmann-like fold, methyltransferase, transferase
• 由来する生物種: Streptomyces carzinostaticus subsp. neocarzinostaticus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 70683.68

構造登録者

Cooke, H.A.,Bruner, S.D. (登録日: 2009-07-03, 公開日: 2009-09-01, 最終更新日: 2023-09-06)

• 主引用文献: Cooke, H.A.,Guenther, E.L.,Luo, Y.,Shen, B.,Bruner, S.D.; Molecular basis of substrate promiscuity for the SAM-dependent O-methyltransferase NcsB1, involved in the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin.; Biochemistry, 48:9590-9598, 2009; PubMed: 19702337; DOI: 10.1021/bi901257q

実験手法

X-RAY DIFFRACTION (2.69 Å)