3I4N

8-oxoguanine containing RNA polymerase II elongation complex E

Summary for 3I4N

• Entry DOI: 10.2210/pdb3i4n/pdb
• Related: 3I4M
• Descriptor: DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerases I, II, and III subunit RPABC5, DNA-directed RNA polymerase II subunit RPB11, ... (17 entities in total)
• Functional Keywords: rna polymerase ii, metal-binding, transcription bubble, elongation complex, transcription, 8-oxoguanine, dna damage, oxidative damage, dna-binding, dna-directed rna polymerase, isopeptide bond, magnesium, nucleotidyltransferase, nucleus, phosphoprotein, transferase, zinc-finger, dna repair, mrna processing, transferase-dna-rna hybrid complex, transferase/dna-rna hybrid
• Biological source: Saccharomyces cerevisiae (baker's yeast); More
• Cellular location: Nucleus: P04050 P38902 P08518 P16370 P20433 P20434 P34087 P20436 P27999; Nucleus, nucleolus: P22139 P40422; Cytoplasm: P20435
• Total number of polymer chains: 15
• Total formula weight: 532306.12

Authors

Damsma, G.E.,Cramer, P. (deposition date: 2009-07-02, release date: 2009-09-08, Last modification date: 2024-10-30)

Primary citation

Damsma, G.E.,Cramer, P.
Molecular basis of transcriptional mutagenesis at 8-oxoguanine
J.Biol.Chem., 284:31658-31663, 2009

PubMed Abstract: Structure-function analysis has revealed the mechanism of yeast RNA polymerase II transcription at 8-oxoguanine (8-oxoG), the major DNA lesion resulting from oxidative stress. When polymerase II encounters 8-oxoG in the DNA template strand, it can misincorporate adenine, which forms a Hoogsteen bp with 8-oxoG at the active center. This requires rotation of the 8-oxoG base from the standard anti- to an uncommon syn-conformation, which likely occurs during 8-oxoG loading into the active site. The misincorporated adenine escapes intrinsic proofreading, resulting in transcriptional mutagenesis that is observed directly by mass spectrometric RNA analysis.

PubMed: 19758983
DOI: 10.1074/jbc.M109.022764

Experimental method

X-RAY DIFFRACTION (3.9 Å)