3I40

Human insulin

Summary for 3I40

• Entry DOI: 10.2210/pdb3i40/pdb
• Related: 3I3Z
• Descriptor: Insulin A chain, Insulin B chain (3 entities in total)
• Functional Keywords: insulin, carbohydrate metabolism, cleavage on pair of basic residues, diabetes mellitus, disease mutation, disulfide bond, glucose metabolism, hormone, secreted
• Biological source: Homo sapiens (human); More
• Cellular location: Secreted: P01308 P01308
• Total number of polymer chains: 2
• Total formula weight: 5787.63

Authors

Timofeev, V.I.,Bezuglov, V.V.,Miroshnikov, K.A.,Chuprov-Netochin, R.N.,Kuranova, I.P. (deposition date: 2009-07-01, release date: 2010-01-05, Last modification date: 2023-11-01)

Primary citation

Timofeev, V.I.,Chuprov-Netochin, R.N.,Samigina, V.R.,Bezuglov, V.V.,Miroshnikov, K.A.,Kuranova, I.P.
X-ray investigation of gene-engineered human insulin crystallized from a solution containing polysialic acid.
Acta Crystallogr.,Sect.F, 66:259-263, 2010

PubMed Abstract: Attempts to crystallize the noncovalent complex of recombinant human insulin with polysialic acid were carried out under normal and microgravity conditions. Both crystal types belonged to the same space group, I2(1)3, with unit-cell parameters a = b = c = 77.365 A, alpha = beta = gamma = 90.00 degrees. The reported space group and unit-cell parameters are almost identical to those of cubic insulin reported in the PDB. The results of X-ray studies confirmed that the crystals obtained were cubic insulin crystals and that they contained no polysialic acid or its fragments. Electron-density maps were calculated using X-ray diffraction sets from earth-grown and microgravity-grown crystals and the three-dimensional structure of the insulin molecule was determined and refined. The conformation and secondary-structural elements of the insulin molecule in different crystal forms were compared.

PubMed: 20208155
DOI: 10.1107/S1744309110000461

Experimental method

X-RAY DIFFRACTION (1.85 Å)