3HWJ

Crystal structure of the second PHR domain of Mouse Myc-binding protein 2 (MYCBP-2)

3HWJ の概要

• エントリーDOI: 10.2210/pdb3hwj/pdb
• 関連するPDBエントリー: 3GBW
• 分子名称: E3 ubiquitin-protein ligase MYCBP2, DIMETHYL SULFOXIDE (3 entities in total)
• 機能のキーワード: myc-binding protein 2, mycbp2, phr proteins, phr domain, probable e3 ubiquitin-protein ligase mycbp2, protein associated with myc, pam, phr1, structural genomics, psi-2, protein structure initiative, new york structural genomix research consortium, nysgxrc, alternative splicing, ligase, metal-binding, nucleus, phosphoprotein, transcription, transcription regulation, ubl conjugation pathway, zinc, zinc-finger, new york sgx research center for structural genomics
• 由来する生物種: Mus musculus (mouse)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 38181.45

構造登録者

Sampathkumar, P.,Ozyurt, S.A.,Wasserman, S.R.,Miller, S.A.,Bain, K.T.,Rutter, M.E.,Gheyi, T.,Klemke, R.L.,Atwell, S.,Sauder, J.M.,Burley, S.K.,New York SGX Research Center for Structural Genomics (NYSGXRC) (登録日: 2009-06-17, 公開日: 2009-07-21, 最終更新日: 2024-10-30)

主引用文献

Sampathkumar, P.,Ozyurt, S.A.,Miller, S.A.,Bain, K.T.,Rutter, M.E.,Gheyi, T.,Abrams, B.,Wang, Y.,Atwell, S.,Luz, J.G.,Thompson, D.A.,Wasserman, S.R.,Emtage, J.S.,Park, E.C.,Rongo, C.,Jin, Y.,Klemke, R.L.,Sauder, J.M.,Burley, S.K.
Structures of PHR domains from Mus musculus Phr1 (Mycbp2) explain the loss-of-function mutation (Gly1092-->Glu) of the C. elegans ortholog RPM-1.
J.Mol.Biol., 397:883-892, 2010

PubMed Abstract: PHR [PAM (protein associated with Myc)-HIW (Highwire)-RPM-1 (regulator of presynaptic morphology 1)] proteins are conserved, large multi-domain E3 ubiquitin ligases with modular architecture. PHR proteins presynaptically control synaptic growth and axon guidance and postsynaptically regulate endocytosis of glutamate receptors. Dysfunction of neuronal ubiquitin-mediated proteasomal degradation is implicated in various neurodegenerative diseases. PHR proteins are characterized by the presence of two PHR domains near the N-terminus, which are essential for proper localization and function. Structures of both the first and second PHR domains of Mus musculus (mouse) Phr1 (MYC binding protein 2, Mycbp2) have been determined, revealing a novel beta sandwich fold composed of 11 antiparallel beta-strands. Conserved loops decorate the apical side of the first PHR domain (MmPHR1), yielding a distinct conserved surface feature. The surface of the second PHR domain (MmPHR2), in contrast, lacks significant conservation. Importantly, the structure of MmPHR1 provides insights into a loss-of-function mutation, Gly1092-->Glu, observed in the Caenorhabditis elegans ortholog RPM-1.

PubMed: 20156452
DOI: 10.1016/j.jmb.2010.02.017

実験手法

X-RAY DIFFRACTION (2.25 Å)