3HT2

Zink containing polyketide cyclase RemF from Streptomyces resistomycificus

3HT2 の概要

• エントリーDOI: 10.2210/pdb3ht2/pdb
• 関連するPDBエントリー: 3HT1
• 分子名称: RemF protein, ZINC ION (3 entities in total)
• 機能のキーワード: cupin fold, zn-binding, antibiotic biosynthesis, resistomycin, metalloprotein, cyclase, lyase
• 由来する生物種: Streptomyces resistomycificus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 33272.84

構造登録者

Silvennoinen, L.,Sandalova, T.,Schneider, G. (登録日: 2009-06-11, 公開日: 2009-10-13, 最終更新日: 2024-11-20)

主引用文献

Silvennoinen, L.,Sandalova, T.,Schneider, G.
The polyketide cyclase RemF from Streptomyces resistomycificus contains an unusual octahedral zinc binding site
Febs Lett., 583:2917-2921, 2009

PubMed Abstract: RemF is a polyketide cyclase involved in the biosynthesis of the aromatic pentacyclic metabolite resistomycin in Streptomyces resistomycificus. The enzyme is a member of a structurally hitherto uncharacterized class of polyketide cyclases. The crystal structure of RemF was determined by SAD and refined to 1.2 A resolution. The enzyme subunit shows a beta-sandwich structure with a topology characteristic for the cupin fold. RemF contains a metal binding site located at the bottom of the predominantly hydrophobic active site cavity. A zinc ion is coordinated to four histidine side chains, and two water molecules in octahedral ligand sphere geometry, highly unusual for zinc binding sites in proteins.

PubMed: 19665022
DOI: 10.1016/j.febslet.2009.07.061

実験手法

X-RAY DIFFRACTION (2 Å)