3HG6

Crystal Structure of the Recombinant Onconase from Rana pipiens

3HG6 の概要

• エントリーDOI: 10.2210/pdb3hg6/pdb
• 分子名称: Onconase, SULFATE ION, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: alpha and beta protein, endonuclease, hydrolase, nuclease
• 由来する生物種: Rana pipiens
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 12321.99

構造登録者

Camara-Artigas, A.,Gavira, J.A.,Casares-Atienza, S.,Weininger, U.,Balbach, J.,Garcia-Mira, M.M. (登録日: 2009-05-13, 公開日: 2010-05-19, 最終更新日: 2024-11-06)

主引用文献

Casares-Atienza, S.,Weininger, U.,Camara-Artigas, A.,Balbach, J.,Garcia-Mira, M.M.
Three-state thermal unfolding of onconase.
Biophys.Chem., 159:267-274, 2011

PubMed Abstract: Onconase is a member of the ribonuclease A superfamily currently in phase IIIb clinical trials as a treatment for malign mesothelioma due to its cytotoxic activity selective against tumor-cells. In this work, we have studied the equilibrium thermal unfolding of onconase using a combination of several structural and biophysical techniques. Our results indicate that at least one significantly populated intermediate, which implies the exposure of hydrophobic surface and significant changes in the environment around Trp3, occurs during the equilibrium unfolding process of this protein. The intermediate begins to populate at about 30° below the global unfolding temperature, reaching a maximum population of nearly 60%, 10° below the global unfolding temperature.

PubMed: 21840114
DOI: 10.1016/j.bpc.2011.07.005

実験手法

X-RAY DIFFRACTION (1.7 Å)