3HC4

BHA10 IgG1 Fab quadruple mutant variant - antibody directed at human LTBR

Summary for 3HC4

• Entry DOI: 10.2210/pdb3hc4/pdb
• Related: 3HC0
• Descriptor: IMMUNOGLOBULIN IGG1 FAB, HEAVY CHAIN, IMMUNOGLOBULIN IGG1 FAB, LIGHT CHAIN, ACETATE ION, ... (5 entities in total)
• Functional Keywords: igg1 fab, bha10, immune system
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 2
• Total formula weight: 46566.41

Authors

Arndt, J.W.,Jordan, J.L.,Lugovskoy, A.,Wang, D. (deposition date: 2009-05-05, release date: 2009-08-04, Last modification date: 2023-09-06)

Primary citation

Jordan, J.L.,Arndt, J.W.,Hanf, K.,Li, G.,Hall, J.,Demarest, S.,Huang, F.,Wu, X.,Miller, B.,Glaser, S.,Fernandez, E.J.,Wang, D.,Lugovskoy, A.
Structural understanding of stabilization patterns in engineered bispecific Ig-like antibody molecules
Proteins, 77:832-841, 2009

PubMed Abstract: Bispecific immunoglobulin-like antibodies capable of engaging multiple antigens represent a promising new class of therapeutic agents. Engineering of these molecules requires optimization of the molecular properties of one of the domain components. Here, we present a detailed crystallographic and computational characterization of the stabilization patterns in the lymphotoxin-beta receptor (LTbetaR) binding Fv domain of an anti-LTbetaR/anti-TNF-related apoptosis inducing ligand receptor-2 (TRAIL-R2) bispecific immunoglobulin-like antibody. We further describe a new hierarchical structure-guided approach toward engineering of antibody-like molecules to enhance their thermal and chemical stability.

PubMed: 19626705
DOI: 10.1002/prot.22502

Experimental method

X-RAY DIFFRACTION (1.62 Å)