3HC3

BHA10 IgG1 Fab double mutant variant - antibody directed at human LTBR

3HC3 の概要

• エントリーDOI: 10.2210/pdb3hc3/pdb
• 関連するPDBエントリー: 3HC0 3HC4
• 分子名称: IMMUNOGLOBULIN IGG1 FAB, LIGHT CHAIN, IMMUNOGLOBULIN IGG1 FAB, HEAVY CHAIN, ACETATE ION, ... (5 entities in total)
• 機能のキーワード: igg1 fab, bha10, immune system
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 46994.96

構造登録者

Arndt, J.W.,Jordan, J.L.,Lugovskoy, A.,Wang, D. (登録日: 2009-05-05, 公開日: 2009-08-04, 最終更新日: 2024-10-16)

主引用文献

Jordan, J.L.,Arndt, J.W.,Hanf, K.,Li, G.,Hall, J.,Demarest, S.,Huang, F.,Wu, X.,Miller, B.,Glaser, S.,Fernandez, E.J.,Wang, D.,Lugovskoy, A.
Structural understanding of stabilization patterns in engineered bispecific Ig-like antibody molecules
Proteins, 77:832-841, 2009

PubMed Abstract: Bispecific immunoglobulin-like antibodies capable of engaging multiple antigens represent a promising new class of therapeutic agents. Engineering of these molecules requires optimization of the molecular properties of one of the domain components. Here, we present a detailed crystallographic and computational characterization of the stabilization patterns in the lymphotoxin-beta receptor (LTbetaR) binding Fv domain of an anti-LTbetaR/anti-TNF-related apoptosis inducing ligand receptor-2 (TRAIL-R2) bispecific immunoglobulin-like antibody. We further describe a new hierarchical structure-guided approach toward engineering of antibody-like molecules to enhance their thermal and chemical stability.

PubMed: 19626705
DOI: 10.1002/prot.22502

実験手法

X-RAY DIFFRACTION (1.72 Å)