The Crystal Structure of C176A Mutated [Fe]-Hydrogenase (Hmd) Holoenzyme in Complex with Methylenetetrahydromethanopterin

Summary for 3H65

Related3F46 3F47 2B0J
Descriptor5,10-methenyltetrahydromethanopterin hydrogenase, 5'-O-[(S)-hydroxy{[2-hydroxy-3,5-dimethyl-6-(2-oxoethyl)pyridin-4-yl]oxy}phosphoryl]guanosine, FE (II) ION, ... (7 entities in total)
Functional Keywordsrossmann fold, helix bundle, complex with iron guanylyl pyridinol cofactor, c176a mutant, complex with methylenetetrahydromethanopterin, methanogenesis, one-carbon metabolism, oxidoreductase
Biological sourceMethanocaldococcus jannaschii (Methanococcus jannaschii)
Total number of polymer chains1
Total molecular weight40288.21
Hiromoto, T.,Warkentin, E.,Shima, S.,Ermler, U. (deposition date: 2009-04-23, release date: 2009-09-01, Last modification date: 2017-11-01)
Primary citation
Hiromoto, T.,Warkentin, E.,Moll, J.,Ermler, U.,Shima, S.
The crystal structure of an [Fe]-hydrogenase-substrate complex reveals the framework for H2 activation.
Angew.Chem.Int.Ed.Engl., 48:6457-6460, 2009
PubMed: 19623593 (PDB entries with the same primary citation)
DOI: 10.1002/anie.200902695
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.210302.5%0.9%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
Download full validation report